| Objective: To study the effect of purariae isoflavone on estrogen level in ovariectomized rats.Method:80 rats were divided into four groups randomly, every group with 20 rats:1.Control group;2.Normal + purariae isoflavone group; 3.Ovariectomized group; 4.Ovariectomized + purariae isoflavone group. Estrogen level and gonadotropin-releasing hormon level of all rats were measured. Result:Thirty days after being ovariectomized , E 2, E 3 level was signifcantly lower than that of the first group(P<0.05) .B... | | 目的 :研究葛根提取物对大鼠雌激素水平的影响。方法 :将 80只成年雌性大鼠随机分成 4组 ,每组2 0只 :正常对照组 ;正常 +灌胃葛根提取物 ,30d ;去势组 ,去势 +灌胃葛根提取物 ,30d。抽血测定血清E0 ,E2 ,E3 ,睾酮等性激素水平 ;FSH ,LH ,PRL ,GH等促性激素水平。结果 :去势后 ,E2 ,E3 显著下降 (P <0 .0 5 ) ;E0 有所下降 ,但差异无显著性 (P >0 .0 5 ) ,睾酮以及FSH ,LH ,PRL ,GH等垂体分泌激素增高 (P <0 .0 5 )。葛根提取物灌注后 ,正常组雌激素和促性激素水平均有不同程度的下降 ;去势动物的E2 ,E3 恢复正常 ;E0 和睾酮比正常组增高 (P <0 .0 5 ) ,但睾酮水平与去势组比较差异无显著性 (P >0 .0 5 )。结论 :葛根提取物可恢复去势大鼠的雌激素水平 ,提高促性激素水平 ;在正常大鼠体内 ,该物质表现为抗雌激素作用。 | | 文摘来源 | | Objective The main idea of this study was to investigate the effect and the position of apigenin on the reproductive axis of female rat in vitro. Methods Adult female S-D rats were injected ACSF,DMSO,10~(-5)mol/L Api,10~(-5)mol/L Api +hCG,through lateral brain ventricle and decapitated to take blood,pituitary and ovary.FSH,LH,E_2 and substance P in serum,pituitary and ovary were determined by radiommunassay. Results(1)The ACSF group wasn't significant deviation than the DMSO group.(2)FSH,LH,E_2 and P of the... | | 目的探讨芹菜素在生殖轴调控中的作用及作用部位。方法通过阴道涂片选用情期成年雌性大鼠50只,随机分为五组,分别侧脑室注入人工脑脊液、二甲基亚砜、芹菜素10-5M、hCG及芹菜素10-5M+hCG,各组再分为3d组、5d组。分别于侧脑室注射后的3d、5d断头取血、卵巢及垂体,应用放射免疫方法分别测定血清、卵巢组织及垂体组织中FSH、LH、E2及P含量,以观察浓度为10-5M的芹菜素对生殖轴的作用。结果1.人工脑脊液组与二甲基亚砜组无明显差异。2.单用10-5M芹菜素组与人工脑脊液组相比FSH、LH、E2、P的含量数值小,其差异有显著性。3.单用hCG组与人工脑脊液组相比FSH、LH的含量数值小,而E2、P的含量数值大,其差异均有显著性。4.10-5M芹菜素与hCG合用组与单用10-5M芹菜素组相比FSH及LH的含量数值小,而E2、P的含量数值大,其差异均有显著性。5.10-5M芹菜素与hCG合用组与单用hCG组相比FSH、LH的含量数值大,而E2、P的含量数值小,其差异均有显著性。结论芹菜素在下丘脑水平发挥作用,进而作用于腺垂体、卵巢。10-5M芹菜素能... | | 文摘来源 | | Objective: To explore the phytoestrogenic effects of ten kinds of Chinese medicine including flos carthami,radix cyathulae,radix salviae miltiorrhizae,fructus ligustri lucidi,fructus lycii,radix clycyrrhizae,herba cistanches,herba epimedii,fructus psoraleae and semen cuscutae.Method: 240 female Kunming mice weighting 9~12 g were randomly divided into two main groups A and B.A group was divided into 12 small groups: 1 solvent control group,1 diethylstilbestrol control group and 10 Chinese medicine groups.B g... | | 目的:评价红花、川牛膝、丹参、女贞子、枸杞、甘草、肉苁蓉、淫羊藿、补骨脂、菟丝子等10味中药的植物雌激素活性。方法:选用昆明种雌性乳鼠,随机分为A,B二组:A组又分为12组(溶剂对照组、己烯雌酚组和10个中药组);B组也分为12组(溶剂对照组、己烯雌酚组和10个中药拮抗组),其中中药拮抗组每天同时灌胃中药和己烯雌酚;各组给药4d后分离血清。利用雌激素受体(ER)阳性MCF-7细胞,以MTT法检测中药含药血清对细胞增殖的影响。结果:A组中,红花、川牛膝、丹参、枸杞、肉苁蓉、淫羊藿、补骨脂和菟丝子等中药含药血清可显著促进MCF-7细胞增殖;而女贞子组可见细胞增殖受到明显抑制。B组中,红花、川牛膝、丹参、甘草、淫羊藿、补骨脂和菟丝子组可见己烯雌酚促细胞增殖的作用受到了显著抑制;而肉苁蓉可协同并显著增强己烯雌酚促细胞增殖的作用。结论:红花、川牛膝、丹参、淫羊藿、补骨脂、菟丝子等6种中药在单独用药时具有拟雌激素作用,在与雌激素同时用药时具有抗雌激素作用,其双向调节效应依赖于体内雌激素水平的高低。 |
|
