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办事情总得有始有终，给老外写信得到的答复，相信这个对大家比较有用吧，拿出来跟大家分享了，呵呵： there is no real major sequence difference between kappa and lambda; the sequence differences are quite subtle. You can usually tell which a sequence is by looking at CDR-L1. In kappa, CDR-L1 generally starts with a sequence like RAS, QAS, RTS, RMS, KSS, RST, SAS. In other words, L24 is normally R or K, sometimes Q or S L25 is normally A, sometimes T, M or S L26 is normally S, occasionally T On the other hand, lambda sequences tend to be SGD, SGG, SGS, GGN, GNN, TGT, TLR, TLS. In other words, L24 is normally S, G or T L25 is normally G, sometimes L L26 is normally D,G,N,S,R or T Also in Kappa, L47 is almost always W or L in Lambda, L47 is I, L, V or M I could go on describing more trends like these, but basically, as with the human heavy chain sub-classes, you have to build up a set of propensities for each residue at each position for lambda and kappa and score your sequence against those profiles to see which it matches better. |
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