?30 ?3ù?d?Vo1.30No.3
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? IóD:
[1]ChangMC.Fertilizationofrabbitovainvitro[J].
Nature,1959,(184):466-467.
[2]BrackettBG,BousquetD,BioceML,etal.,Nor-
maldevelopmentfollowinginvitrofertilization[J].in
cow.Biol.Reprod.,1982,(27):147-158.
[3]LuKH,GordonI,McGovernH,etal.Birthof
twinsaftertransferofcattleembryosproducedbyin
vitrotechnique[J].VetRepord.,1988,(122):539-
540.
[4]SaekiK,HoshiM,Leibfried-RutledgeML.Invitro
fertilizationanddevelopmentofbovineoocytesma-
turedinserum-freemedium[J].BiolReprod.,1991,
(44):256-260.
[5]
>6
Hf¤.d
x?
x%8??
?￥ù?[J].
j<vDD,1999,27(2):17-20.
[6]BekerARCL.,ColenbranderB,BeversMM.Effectof
17β-estradiolontheinvitromaturationofbovine
oocytes[J].Theriogenology,2002,(58):1663-
1673.
EffectofPVPand17β-E2onInVitroMaturationandFertilization
ofBovineOocytes
XIAOZheng-zhong1,LAISong-jia2,WUSu-xiao1
(1.DepartmentofAgriculturalEngineering,GuangdonyShaoguanCollege,Shaoguan,Guangdong512005,China;
2.CollegeofAnimalScienceandTechnology,SichuanAgriculturalUniversity,Yaan,Sichuan625014,China)
Abstract:With0.3%PVPsubstitutingfor10%FBSculturemediaforIVM,thematurationandfer-
tilizationrateofoocyteswerenotsignificantlydifferentbetweenyellowcattleandbuffalo(P>0.05).The
resultsindicatedthat0.3%PVPsubstitutingfor10%FBSwasfeasibleinculturemedia.Adding17β-E2in
culturemediaforIVMofyellowcattleandbuffalooocytes,thematurationrateandfertilizationratewere
bothsignificantlyhigherthanthosewithoutadding17β-E2(P<0.01orP<0.05).Theresultsshowedthat
17β-E2wasindispensabletothematurationandinvitrofertilizationofbovineoocytes.Atthesametime,
theaimofthisstudywastoelucidatethepossibilityofapplyingbovineIVFprocedureinbuffalo.There-
sultsshowedthatalthoughbuffalooocytescouldmatureandfertilizeinvitroculture,thematurationrate
andfertilizationrateweresignificantlylowerthanthoseofyellowcattleandHolsteincowoocytesunder
theidenticalconditions(P<0.05orP<0.01),whichshowedthatapplicationofIVFprocedureofyellow
cattleisefficientinbuffalo,butfertilizationmediaorcertainstepsofthisproceduredoesnotreallysuitfor
buffalo,itstillneedstobefurtherimproved.
Keywords:PVP;17β-E2;Oocyte;Invitromaturation;Invitrofertilization.
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