【专题讨论】细胞划痕实验

Gut. 2007 Jan;56(1):95-106.  (sci Impact Factor 9.002  )
Thompson CC, Ashcroft FJ, Patel S, Saraga G, Vimalachandran D, Prime W, Campbell F, Dodson A, Jenkins RE, Lemoine NR, Crnogorac-Jurcevic T, Yin HL, Costello E.Pancreatic cancer cells overexpress gelsolin family-capping proteins, which contribute to their cell motility. Gut. 2007 Jan;56(1):95-106.  (sci Impact Factor 9.002  )

方法：In vitro wound-healing assay
Panc-1 and Suit-2 cells were treated with siRNA as described above. After incubation for 72 (CapG) or 48 (Gelsolin) h, the cells were removed by trypsinisation, counted and plated at 400000 cells/ml in 12-well dishes. Cells were incubated overnight yielding confluent monolayers for wounding. Wounds were made using a pipette tip and photographs taken immediately (time zero) and 12 or 16 h after wounding for Suit-2 and Panc-1 cells, respectively. The distance migrated by the cell monolayer to close the wounded area during this time period was measured. Results were expressed as a migration
index—that is, the distance migrated by siRNA treated (control or targeted) relative to the distance migrated by RISC-free control RNA treated cells. Experiments were carried out in triplicate and repeated at least five times.