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Original Article The effect of hemolysis on 41 chemistry and immunology tests and determination of hemolysis alert index Xia Liangyu, Xu Ermu, Cao Xince, Liu Li, Liu Qian, Cheng Xinqi, Hu Yingying, Zhang Lin, Qiu Ling Published on 2017-12-11 Cite as Chin J Lab Med, 2017,40(12): 947-952. DOI: 10.3760/cma.j.issn.1009-9158.2017.12.009 Abstract Objective To evaluate hemolyticinfluence on 41 chemistry and immunology tests and define the hemolysis alert index, which can be used as evidence for sample rejection and test report verification. Methods Method evaluation. As a pair, both hemolyzed and non-hemolyzed serum samples were collected from the same patient at the same time. Hemolysis index and 41 tests were analyzed. The effect of hemolysis in different hemolysis level was evaluated by comparing results of the paired samples. The alertindex were defined by senior laboratory techniciansand clinical professionals based on discussion of widely accepted quality requirements. Results The number of sample with hemolysis index of 1, 2, 3 and 4was 24, 17, 7 and 4 respectively. Of the 41 analytes, 6 analytes increased in hemolyzed samples comparing to non-hemolyzed samples, namelyaspartate aminotransferase(AST), creatine kinase(CK), potassium(K), lactate dehydrogenase(LD), inorganic phosphorus(iP) and total bilirubin(TBil). Another 9 analytesdecreased in hemolyzed samples comparing to non-hemolyzed samples. These analytes are alkaline phosphatase(ALP), chloride(Cl), creatinine(Cr), immunoglobin M(IgM), sodium(Na), prealbumin(PA), rheumatoid factor(RF), triglyceride(TG), uric acid(UA). Hemolysis alert index was defined as 1 for AST, K, Na and LD; 2 for Cr; 3 for CK, iP, PA and TBil; and 4 for the other 32 tests. Conclusions Hemolysisalertindex were defined based on the investigation of hemolyticinfluence on 41 chemistry and immunology tests in ourlaboratory. However, hemolytic influence on clinical laboratory tests are closely related to the assay systems. So clinical laboratory should evaluate the hemolytic influence on its own analysis system, and define assay specific hemolysis alert index.(Chin J Lab Med, 2017, 40: 947-952) Key words: Hemoglobins; Hemolysis; Specimen handling; Clinical chemistry tests; Immunoassay; Evaluation studies Contributor Information Xia Liangyu Department of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China Xu Ermu Cao Xince Liu Li Liu Qian Cheng Xinqi Hu Yingying Zhang Lin Qiu Ling |
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来自： 雨偌yu > 《溶血对生化的影响》
