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在这个世界上将越来越依赖有效的植物生长来获取足够的食物的世界中，了解植物激素作用的天然机制非常重要。 在这项工作中，向生长素分子中引入荧光团是一种敏感且非侵入性的方法，可以以高时空分辨率直接可视化生长素的定位。 遗传和化学生物学分析的最新多学科方法与活细胞成像，液相色谱-质谱（LC-MS）和表面等离振子共振（SPR）方法一起用于生长素相关生物学的表征 化合物在拟南芥中的活性，分布和稳定性。 尽管在体内有部分代谢，这些荧光生长素仍显示出不均匀和动态分布，导致在已知浓缩天然生长素的组织（如根尖钩的凹面）中形成最大荧光。 图 1 FluorAs筛选策略 重要的是，它们的分布会因根和芽中不同的外源刺激而改变。 此外，我们也鉴定了存在于内质网和内体中的荧光生长素类似物的亚细胞定位情况。 我们的工作提供了强大的工具，可在细胞或亚细胞水平上可视化植物生长素在不同植物组织中的分布，并在植物发育过程中响应内部和环境刺激。 In a world that will rely increasingly on efficient plant growth for sufficient food, it is important to learn about natural mechanisms of phytohormone action. In this work, the introduction of a fluorophore to an auxin molecule represents a sensitive and non‐invasive method to directly visualise auxin localisation with high spatiotemporal resolution. The state‐of‐the‐art multidisciplinary approaches of genetic and chemical biology analysis together with live cell imaging, liquid chromatography–mass spectrometry (LC‐MS) and surface plasmon resonance (SPR) methods were employed for the characterisation of auxin‐related biological activity, distribution and stability of the presented compounds in Arabidopsis thaliana. Despite partial metabolisation in vivo, these fluorescent auxins display an uneven and dynamic distribution leading to the formation of fluorescence maxima in tissues known to concentrate natural auxin, such as the concave side of the apical hook. Importantly, their distribution is altered in response to different exogenous stimuli in both roots and shoots. Moreover, we characterised the subcellular localisation of the fluorescent auxin analogues as being present in the endoplasmic reticulum and endosomes. Our work provides powerful tools to visualise auxin distribution within different plant tissues at cellular or subcellular levels and in response to internal and environmental stimuli during plant development. New fluorescent auxin probes visualise tissue‐specific and subcellular distributions of auxin in Arabidopsis10.1111/nph.17183  文章来源原创，转载请注明来源。 温馨提示： 为方便PaperRSS粉丝们科研、就业等话题交流。我们根据10多个专业方向（植物、医学、药学、人工智能、化学、物理、财经管理、体育等），特建立了30个国内外博士交流群。群成员来源欧美、日韩、新加坡、清华北大、中科院等全球名校。 |
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