|
花青素有助于植物着色，是人类饮食中的抗氧化剂的宝贵来源作为水果和蔬菜的组成部分。 众所周知，花青素的产生是由光在苹果果实中诱导的 然而，负责早期光诱导的花青素生物合成的潜在的分子机制仍然尚不清楚。 在这里，我们鉴定了一种ERF（乙烯响应因子）蛋白，ERF109，参与光诱导的花青素生物合成，并且发现它通过直接与花青素相关的基因启动子直接结合着色。 启动子:: GUS（β-葡萄糖醛酸酶）报告分析和Hi-C测序显示，长期非编码RNA（LNCRNA），MDLNC499位于MDERF109上游，诱导MDERF109的表达。 发现MDLNC499启动子中的W型盒CIS-元素被转录因子MDWRKY1调节。 苹果果实中的瞬态表达且苹果Calli的稳定变换允许我们重建MDWRKY1-MDLNC499-MDRF109转录级联，其中MDWRKY1被光激活，以增加MDLNC499的转录，这反过来诱导MDERF109。 MDERF109蛋白诱导苹果着色早期阶段的花青素相关基因的表达和花青素的积累。 我们的结果提供了一个更好地理解光诱导的苹果果实着色的各种监管机制的平台。 Anthocyanin pigments contribute to plant coloration and are valuable sources of antioxidants in the human diet as components of fruits and vegetables. Their production is known to be induced by light in (Malus domestica) apple fruit; however, the underlying molecular mechanism responsible for early-stage light-induced anthocyanin biosynthesis remains unclear. Here, we identified an ERF (ethylene response factor) protein, ERF109, involved in light-induced anthocyanin biosynthesis and found that it promotes coloration by directly binding to anthocyanin-related gene promoters. Promoter::GUS (β-glucuronidase) reporter analysis and Hi-C sequencing showed that a long non-coding RNA (lncRNA), MdLNC499, located upstream from MdERF109, induces the expression of MdERF109. A W-box cis-element in the MdLNC499 promoter was found to be regulated by a transcription factor, MdWRKY1. Transient expression in apple fruit and stable transformation of apple calli allowed us to reconstruct a MdWRKY1-MdLNC499-MdERF109 transcriptional cascade in which MdWRKY1 is activated by light to increase the transcription of MdLNC499, which in turn induces MdERF109. The MdERF109 protein induces the expression of anthocyanin-related genes and the accumulation of anthocyanins in the early stages of apple coloration. Our results provide a platform for better understanding the various regulatory mechanisms involved in light-induced apple fruit coloration. https://academic./plcell/advance-article-abstract/doi/10.1093/plcell/koab188/6322998?redirectedFrom=fulltext  文章来源网络整理；如有侵权请及时联系PaperRSS小编删除，转载请注明来源。 温馨提示： 为方便PaperRSS粉丝们科研、就业等话题交流。我们根据10多个专业方向（植物、医学、药学、人工智能、化学、物理、财经管理、体育等），特建立了30个国内外博士交流群。群成员来源欧美、日韩、新加坡、清华北大、中科院等全球名校。 |
|
|
