To investigate the molecular mechanism underlying the oncogenic role of PRC1 in lung adenocarcinoma, we utilized NGS to assess the gene expression profiles of PRC1-depleted A549 cells and control cells.
This un- biased genome-scale analysis identified 10,037 differen- tially expressed genes (DEGs) [log2(FoldChange)] > 1 and P < 0.05) in A549 cells after PRC1 knockdown com- pared to controls.
The pathway analysis showed that the Wnt/β-catenin, TGF- β, Hippo, p53, MAPK and cell cycle pathways were significantly enriched from downregulated DEGs (Fig. 8b and c).
The Wnt/β-catenin pathway was the most significantly dysregulated in re- sponse to PRC1-depeletion.
An expression heatmap of 44 Wnt/β-ca- tenin pathway relevant DEGs after the transduction of shPRC1–1 is shown in Fig. 8c.