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Synthesis and structure Antonia Red™ dextrans are synthesised from well-characterized dextran fractions derived from Leuconostoc mesenteroides labelled with Antonia Red. After purification from non-bound dye, the products are controlled for Mw, appearance, solubility, DS, fluorescence and free dye. The products are designated by the approximate molecular weights of the dextran fractions used. Thus, for example, the product Antonia Red dextran 4 has a molecular weight of approx. 4000 Da. The actual molecular weight is determined by GPC. This value is supplied with the Certificate of Analysis. The dextran used is from Leuconostoc mesenteroides B-512F which is essentially a linear α-(1-6)-linked glucose chain with however a low percentage (2-5%) of α-(1-3) branches distributed along the chain. The dextran fractions used are from Mw of 4000 to 150000 and are carefully controlled by GPC, optical rotation, absorbance and other control parameters.
Antonia Red-葡聚糖结构特征 Spectral properties Antonia Red dextran has an absorbance maximum at 583 nm (in borate buffer, pH 9.0) and an emission wavelength of 602 nm. The dye residue exhibits high photo- and chemical stability and bright fluorescence. The DS lies between 0.001-0.01. Stability No prospective stability studies on Antonia Red-dextrans have been performed. However, the structural properties of the dextran carrier and those of the chemical linkage of the Antonia Red-dye to the dextran chain would suggest good stability of the product. It is recommended that the products are stored in air-tight containers in the dark. Antonia Red dextrans may be stored at ambient temperatures. Applications Antonia Red dextrans are primarily used for studying permeability and transport in cells and tissues, trace cell lineage and perform vasculature studies. An added benefit is that measurements of the fluorescence provide quantitative data on the permeability of healthy and diseased tissues. Such studies can be performed in real time by intra-vital fluorescence microscopy. The technique offers high sensitivity and concentrations down to 1μg/ml can be detected in tissue fluids. Antonia Red dextrans is brightly fluorescent using the red (Texas Red) filter in fluorescence microscopy Antonia Red-葡聚糖#ARD4/ARD20/ARD40/ARD150, 为紫色粉末，易溶于水或电解质溶液。它们主要用于研究细胞和组织中的渗透性和转运、细胞谱系示踪和脉管系统研究。 Antonia Red-葡聚糖是由来源于用Antonia红标记的间质明串珠菌。从非结合染料中纯化后控制产品的分子量、外观、溶解度、DS、荧光和游离染料。产品由所用葡聚糖级分的近似分子量表示。因此，对于例如，产品Antonia Red右旋糖酐4的分子量约为4000Da 分子量通过GPC测定。此值随分析证书一起提供。这个所用的右旋糖酐来自间质明串珠菌B-512F，其本质上是线性α-（1-6）连接的然而，沿链分布的α-（1-3）分支的百分比较低（2-5%）。所使用的葡聚糖级分的Mw为4000至150000，并通过GPC、光学旋转、吸光度和其他控制参数。 应用： Antonia Red-葡聚糖主要用于研究细胞和组织中的渗透性和转运，追踪细胞谱系并进行脉管系统研究。另一个好处是荧光提供了关于健康和患病组织的渗透性的定量数据。这样的研究可以通过活体内荧光显微镜实时进行。该技术提供在组织液中可以检测到高灵敏度和低至1μg/ml的浓度。安东尼娅·雷德在荧光显微镜中使用红色（得克萨斯红）滤光片，葡聚糖具有明亮的荧光。 Antonia Red-葡聚糖相关研究： ARD4 Antonia Red-dextran 4 4000 10mg ARD4 Antonia Red-dextran 4 4000 50mg ARD20 Antonia Red-dextran 20 20000 10mg ARD20 Antonia Red-dextran 20 20000 50mg ARD40 Antonia Red-dextran 40 40000 10mg ARD40 Antonia Red-dextran 40 40000 50mg ARD150 Antonia Red-dextran 150 150000 10mg ARD150 Antonia Red-dextran 150 150000 50mg |
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