抗PI(4)P主要与指示的磷酸肌醇(合成或天然来源)的头部基团反应,并根据测定形式显示与其他磷酸肌醇或磷脂的低交叉反应性。 纯化的抗磷脂酰肌醇4-磷酸(PtdIns(4)P)单克隆抗体。 亚类:IgM 来源:小鼠 应用:ELISA(酶联免疫吸附试验)、免疫荧光、荧光偏振、蛋白-脂质叠加(PIP条带) 分类:抗体与蛋白质 筛选器:抗体、脂质结合、PI(4)P 运输温度:干冰 存储:2-8°C持续30天,-20°C持续超过30天。不要多次冻融 PI(4)P抗体特性: 形态 液体 储存说明 4°C下储存最多30天。如果需要更长时间储存,分装后储存在-20°C。避免反复冻融循环。 储存缓冲液 PBS(磷酸盐缓冲液),pH 7.4 浓度 0.5 毫克/毫升 纯度 使用Protein G琼脂糖亲和纯化 免疫原 合成的PI(4)P(磷脂酰肌醇4-磷酸) 克隆性 单克隆;克隆号PI4-2 类型 IgM(免疫球蛋白M) 数据:免疫荧光 图像已获得Gerald Hammond的许可进行改编。共聚焦图像显示了用抗PI(4)P染色的细胞(左侧)或在染色前预先与PI(4)P预孵化的细胞(右侧)。DAPI对细胞核进行对比染色。 PI(4)P抗体出版物中的特色: 1) Weber, S. S., C. Ragaz, et al. (2006). “Legionella pneumophila exploits PI(4)P to anchor secreted effector proteins to the replicative vacuole.” PLoS Pathog 2(5): e46. 2) Blagoveshchenskaya, A., F. Y. Cheong, et al. (2008). “Integration of Golgi trafficking and growth factor signaling by the lipid phosphatase SAC1.” J Cell Biol 180(4): 803-12. 3) Cheong, F. Y., V. Sharma, et al. (2010). “Spatial Regulation of Golgi Phosphatidylinositol-4-Phosphate is Required for Enzyme Localization and Glycosylation Fidelity.” Traffic 11(9): 1180-1190. 4) Bojjireddy, N., J. Botyanszki, et al. “Pharmacological and Genetic Targeting of the PI4KA Enzyme Reveals Its Important Role in Maintaining Plasma Membrane Phosphatidylinositol 4-Phosphate and Phosphatidylinositol 4,5-Bisphosphate Levels.” Journal of Biological Chemistry 289(9): 6120-6132. PI(4)P抗体相关产品: PI(4)P Mass ELISA K-4000E PI4-Kinase Activity Assay K-4000K PI(4)P Beads P-B004a PI(4)P P-4008,P-4016 BODIPY-FL,TMR PI(4)P C-04F6a,C-04M6a |
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