Molecular Depot：辣根过氧化物酶生物素化，可扩增免疫测定信号

Molecular Depot-辣根过氧化物酶（HRP）是生物化学中应用Z广泛的酶之一。它使用过氧化氢作为氧化剂催化多种底物的氧化。生物素化的HRP可用于扩增某些免疫测定的信号。

Molecular-Depot-辣根过氧化物酶生物素化：

目录编号：P2010010

包装规格：1毫克

比活性：350 U/毫克

单位定义：在20°C的反应缓冲液中，产生1微摩尔的普鲁士蓝素从焦性没食子的生物素化辣根过氧化物酶所需的量，反应缓冲液组成为：8毫摩尔过氧化氢，14毫摩尔磷酸钾，0.5%焦性没食子，pH 6.0，反应时间20秒。

生物素结合量：1.3摩尔生物素/摩尔辣根过氧化物酶（HABA测定法）

外观：冻干粉末

储存：-20°C或更低。避免反复冻融。

纯度：>95%。

推荐缓冲液：10毫摩尔柠檬酸，pH 6.0

Molecular Depot-辣根过氧化物酶生物素化文献参考：

False biochemical diagnosis of hyperthyroidism in streptavidin-biotin-based immunoassays: the problem of biotin intake and related interferences. Piketty ML, Polak M, Flechtner I, Gonzales-Briceño L, Souberbielle JC. Clin Chem Lab Med. 2017 May 1;55(6):780-788.

Characterization of the scope and magnitude of biotin interference in susceptible Roche Elecsys competitive and sandwich immunoassays. Trambas C, Lu Z, Yen T, Sikaris K. Ann Clin Biochem. 2018 Mar;55(2):205-215.

Depletion of biotin using streptavidin-coated microparticles: a validated solution to the problem of biotin interference in streptavidin-biotin immunoassays. Trambas C, Lu Z, Yen T, Sikaris K. Ann Clin Biochem. 2018 Mar;55(2):216-226.

Current good manufacturing practices-compliant manufacture and measurement of biotin-labeled red blood cells. Donnenberg AD, Kanias T, Triulzi DJ, Dennis CJ, Moore LR, Meyer EM, Sinchar D, Kiss JE, Normolle DP, Gladwin MT. Cytotherapy. 2019 Jul;21(7):793-800.

Characterization of biotin interference in 21 Vitros 5600 immunoassays and risk mitigation for patient safety at a large academic medical center. Stieglitz HM, Korpi-Steiner N. Clin Biochem. 2020 Jan;75:53-61.

Availability of NHS-biotin labeling to identify free protein lysine revealed by experiment and MD simulation. Muraoka A, Matsuura Y, Naitow H, Ihara M, Kunishima N. Anal Biochem. 2018 Sep 15;557:46-58.

Biotin accounts for less than half of all biotin and biotin metabolites in the cerebrospinal fluid of children. Bogusiewicz A, Stratton SL, Ellison DA, Mock DM. Am J Clin Nutr. 2008 Nov;88(5):1291-6.

Endogenous biotin in rat brain: implications for false-positive results with avidin-biotin and streptavidin-biotin techniques. McKay BE, Molineux ML, Turner RW. Methods Mol Biol. 2008;418:111-28.