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Table2.CompatiblesubstanceconcentrationsintheBCAProteinAssay(seetextfordetails).
CompatibleCompatible
SubstanceConcentrationSubstanceConcentration
Salts/BuffersDetergents
?
ACES,pH7.825mMBrij-355.0%
Ammoniumsulfate1.5MBrij-56,Brij-581.0%
Asparagine1mMCHAPS,CHAPSO5.0%
Bicine,pH8.420mMDeoxycholicacid5.0%
Bis-Tris,pH6.533mMOctylβ-glucoside5.0%
Borate(50mM),pH8.5(#28384)undiluted
NonidetP-40(NP-40)5.0%
?
B-PERReagent(#78248)undiluted
5.0%
Octylβ-thioglucopyranoside
CalciumchlorideinTBS,pH7.210mM
SDS5.0%
?
Na-Carbonate/Na-Bicarbonate(0.2M),pH9.4undiluted
Span201.0%
(#28382)?
TritonX-1005.0%
Cesiumbicarbonate100mM
TritonX-114,X-305,X-4051.0%
CHES,pH9.0100mM
?
Tween-20,Tween-60,Tween-805.0%
Na-Citrate(0.6M),Na-Carbonate(0.1M),pH9.01:8dilution?
Zwittergent3-141.0%
(#28388)
Chelatingagents
Na-Citrate(0.6M),MOPS(0.1M),pH7.5(#28386)1:8dilution
EDTA10mM
CobaltchlorideinTBS,pH7.20.8mM
EGTA--------
EPPS,pH8.0100mM
Sodiumcitrate200mM
FerricchlorideinTBS,pH7.210mM
Reducing&Thiol-ContainingAgents
Glycine?HCl,pH2.8100mM
N-acetylglucosamineinPBS,pH7.210mM
Guanidine?HCl4M
Ascorbicacid--------
HEPES,pH7.5100mM
Cysteine--------
Imidazole,pH7.050mM
Dithioerythritol(DTE)1mM
MES,pH6.1100mM
Dithiothreitol(DTT)1mM
MES(0.1M),NaCl(0.9%),pH4.7(#28390)undiluted
Glucose10mM
MOPS,pH7.2100mM
Melibiose--------
ModifiedDulbecco’sPBS,pH7.4(#28374)undiluted
2-Mercaptoethanol0.01%
NickelchlorideinTBS,pH7.210mM
Potassiumthiocyanate3.0M
PBS;Phosphate(0.1M),NaCl(0.15M),undiluted
pH7.2(#28372)Thimerosal0.01%
PIPES,pH6.8100mMMisc.Reagents&Solvents
RIPAlysisbuffer;50mMTris,150mMNaCl,undiluted
Acetone10%
0.5%DOC,1%NP-40,0.1%SDS,pH8.0
Acetonitrile10%
Sodiumacetate,pH4.8200mM
Aprotinin10mg/L
Sodiumazide0.2%
DMF,DMSO10%
Sodiumbicarbonate100mM
DMSO10%
Sodiumchloride1M
Ethanol10%
Sodiumcitrate,pH4.8orpH6.4200mM
Glycerol(Fresh)10%
Sodiumphosphate100mM
Hydrazides--------
Tricine,pH8.025mM
Hydrides(NaBHorNaCNBH)--------
243
Triethanolamine,pH7.825mM
HydrochloricAcid100mM
Tris250mM
Leupeptin10mg/L
TBS;Tris(25mM),NaCl(0.15M),pH7.6(#28376)undiluted
Tris(25mM),Glycine(192mM),pH8.0(#28380)1:3dilution
Dilutedwithultrapurewater.
Detergentsweretestedusinghigh-purityThremoScientificSurfact-AmpsProducts,whichhavelowperoxidecontent.
--Dashed-lineentryindicatesthatthematerialisincompatiblewiththeassay.
§
Foramoreextensivelistofsubstances,downloadTechTip#68:ProteinAssayCompatibilityTablefromourwebsite.This
TechTipincludescompatiblesubstancesforallofourproteinassaysandenableseasycomparisons.
PierceBiotechnologyPOBox117(815)968-0747www.thermoscientific.com/pierce
3747N.MeridianRoadRockford,lL61105USA(815)968-7316fax
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