TM
MULTISCREENSTABLECELLLINE
HUMANRECOMBINANTM1RECEPTOR
Datasheet
PRODUCTINFORMATION
Background:ThemuscarinicM1receptorisamembraneproteinwithseven
transmembranesegmentsandbindswithacetylcholineontheextracellularsurface.The
CatalogNumber:C1022-1a
acetylcholine-boundreceptorinteractswithandactivatesGproteinGq/G11.Thereceptor
isfoundinthehippocampalandcorticalregionsofthebrainaswellasinthe
LotNumber:C1022-1a-072309
parasympatheticganglia.Itisinvolvedinmanyprocesses,includingtheinitiationof
6
Quantity:1vial(2x10)frozencells
seizures,learningandmemory,andregulationoftheforceandrateofheartcontractions.
Becauseofitsinvolvementintheseprocesses,theM1receptorisacompellingdrug
FreezeMedium:SigmaFreezing
targetforAlzheimer’sdiseaseandotherneurologicalandpsychiatricdisorders.
Medium(C-6164)
Hostcell:CHO-K1
Application:Functionalassays
Transfection:ExpressionvectorFigure1
containingfull-lengthhumanCHRM1
cDNA(GenBankAccessionNumber
80
-10
NM_000738)withFLAGtagsequence
EC=5.4x10M
50
atN-terminus
60
RecommendedStorage:Liquid
nitrogenuponreceiving40
PropagationMedium:DMEM/F12,
20
10%FBS,10μg/mLpuromycin
Stability:Stableinculturefor
0
-13-12-11-10-9-8-7-6
minimumoftwomonths
Log[Acetylcholine]M
Figure2
Figure1.Dose-dependentstimulationofcalciumfluxupontreatmentwithligand,
monitoredwithFlexStation.Figure2.Receptorexpressiononcellsurfacemeasuredby
flowcytometry(FACS)usingananti-FLAGantibody.Thinline:parentalcells;thickline:
receptor-expressingcells.
References:
Francisetal.(1999)ThecholinergichypothesisofAlzheimer’sdisease:areviewof
progress.JNeurolNeurosurgPsychiatry66:137-147
Kuboet.al.(1986)Cloning,sequencingandexpressionofcomplementaryDNAencoding
themuscarinicacetylcholinereceptor.Nature323:411-416.
FORRESEARCHUSEONLY.
2005MultispanInc.Allrightsreserved.Nopartofthisdocumentmaybereproducedinanyformwithoutpriorpermissioninwriting.
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