Biochemicalinvestigationandgeneanalysisofequol:Aplantandsoy-derived
isoflavonoidwithantiagingandantioxidantpropertieswithpotentialhuman
skinapplications.
RemonaGopaul1,?,,HelenE.Knaggs1,EdwinD.Lephart2
Articlefirstpublishedonline:27JAN2012
Keywords:
?equol;
?extracellularmatrix;
?geneexpression;
?humanskin
Abstract
Thepurposeofthisstudywastoinvestigatetheeffectsofequol,aplantandintestinalfloraderived
isoflavonoidmoleculeontheexpressionofskingenesandproteinsusinghumandermalmodels.As
equolhasbeenshowntomimic17β-estradiolandbindspecificallyto5α-dihydrotestostone(5α-
DHT),theseagentswereused(inadditiontoequol)todeterminewhetherequolmayplayimportant
andbeneficialrolesintheextracellularmatrix(ECM).Equolat0.3or1.2%inqPCRexperiments
usingahumanskinbarriermodelexaminedECMgeneexpression.Equol,5α-DHT,and17β-
estradiolat10nMwerestudiedinhumanmonolayerfibroblastscultures(hMFC)forECMprotein
expression.Humanfibroblastthree-dimensionalorganotypicculturesrevealedequol''sinfluence(@
10nM)onECMproteinsviafluorescent-activatedcellsorting(FACS)analysis.InqPCR
experiments,equolsignificantlyincreasedcollagen,elastin(ELN),andtissueinhibitorof
metalloproteaseanddecreasedmetalloproteinases(MMPs)geneexpressionandcausedsignificant
positivechangesinskinantioxidantandantiaginggenes.InhMFC,equolsignificantlyincreased
collagentypeI(COL1A1),whereas,5α-DHTsignificantlydecreasedcellviabilitythatwasblocked
byequol.FACSanalysisshowedequoland17β-estradiolsignificantlystimulatedCOL1A1,collagen
typeIII(COL3A1),andELNwhileMMPsweresignificantlydecreasedcomparedwithcontrol
values.Finally,tamoxifenblockedthepositiveinfluencesofequolonECMproteinsviaFACS
analysis.Thesefindingssuggestthatequolhasthepotentialtobeusedtopicallyforthetreatmentand
preventionofskinaging,byenhancingECMcomponentsinhumanskin.
HowtoCite
Gopaul,R.,Knaggs,H.E.andLephart,E.D.(2012),Biochemicalinvestigationandgeneanalysisof
equol:Aplantandsoy-derivedisoflavonoidwithantiagingandantioxidantpropertieswithpotential
humanskinapplications.BioFactors.doi:10.1002/biof.191
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