辣木籽对大鼠糖尿病脑病的神经保护

?!.v
?Uh7h￥
ü1T¨


>nullb1,|￥
ü2,·null33,???1,?=￡2,?null′3

(1null浙江省中医药研究院中医临床研究所,浙江杭州310007;2null天津医科大学公共卫生学院,天津300070;

3null中国医学科学院放射医学研究所,天津300192)

nullnullKnull1:目的初步探讨辣木籽对糖尿病脑病的神经保护作用b方法将大鼠随机分为正常组a模型组和辣

木籽组b除正常组外,模型组和辣木籽组腹腔注射链脲佐菌素65mg/kg复制糖尿病脑病模型b辣木籽组灌胃

给予辣木籽0null2g/kg,其余两组给予等体积空白溶媒b连续给药8周b测试学习记忆功能a血糖a血清胰岛素

水平和海马神经元损伤b结果与模型组比较,辣木籽组血糖明显降低,认知障碍明显改善,神经元损伤亦见明

显好转b结论辣木籽对糖尿病脑病表现良好的神经保护作用,颇具进一步研究前景b

nullnull1oM:糖尿病脑病;认知功能障碍;胀亡;辣木籽;大鼠

nullnull?ms ?|:R285null5nullnullnullnullnullóDS?
?:AnullnullnullnullnullócI|:1007-4813(2010)02-0179-02

nullá[":浙江省中医药研究院院内基金项目(2009001)

nullT?eo:刘null冰(1969-),医学博士,师从张伯礼院士b研究方向:神经退行性疾病中医药防治b

nullnull ?!(Moringaoleifera)1 ?! S ?!
?±t,e

á??#dY,Uh??%hμ
üA


r,? ?!]íUh7h?nM1?b''43 ?

!.ó?O?
í
￡üaa???p??
üí
?


￥?Y,C??/b

1null?
DZE

1null1null
L?tnullWistar|?v
?,8×280~320g,

??Y ??
L?t/
?μK
3,?ì￡I|

SCXK(?)2002-2003b

1null2null0tDNnull y?Q )
í(streptozocin,STZ,
?

Ssigma
3), ?!.(0
ê?c ?!
J?μK3?


3ò<),O?
íb
?
k4?(???
3t/
?ù

?
?),×?F?óN(
?S<
3
3),Morris
￡


ù?S eN(?SDD SDy0tù?
?)b

1null3null?ˉ?DsFnull|v
?20o,STZ65mg/kg

(¨0null1mmoLpH4null5^
?
??
A·3)′8?
/

b/a4d,ì
J8h,?
kóbóv?

8null0mmoL/L1?ì?v
?b|
?s1??

?!.
Fb
6|v
?10o,?
?8^
?
??


A,
!1?èFb

1null4nulló0null1?8d 7
S,òF?t??/4
ó

íM?0t:?èF#?F(1%?J8?
í),

1mL), ?!.F( ?!.,ù?%x,[
a


1%?J8?
í)??
?A,0null2g/kg),òF ( ?

?ó08?b

1null5nullMorris
￡
ùnull I3?v
?a???M?#

V1nullv
?ób9?%?a?2?
￥?Y(nullxnulls)

FnullnullnullnullnullY4
/(g/kg)TC/(mmol/L)TG/(mmol/L)

?
<
￡F-1null19null0null270null45null0null32

ú??vF-2null84null0null430null83null0null38

,1§9?4
F0null172null36null0null56#null0null30null0null31null

,1§9?4
F0null342null29null0null48#null0null37null0null22null

,1§9ú4
F0null682null47null0null55#null0null68null0null65null

nullnull?:D baF1?,#P<0null05,D?F1?,nullP<0null05b

V2nulll
?′8

`%}
`??￥?Y(nullxnulls)

FnullnullY4
/(g/kg)OD′/570nm

baF-0null148null0null13null

,1§9?4
F0null240null124null0null08null

,1§9?4
F0null480null246null0null11#

,1§9ú4
F0null960null233null0null10#

nullnull?:D baF1?,#P<0null05b

3null)
?

''
L2TV
ü,ú??vFv
?D?
<
￡

Fv
?M1óbTCaTG (A÷
6úbYY4t

?4
F V[
üA??ú?v
?óbTCaTG,ii

B?￥
r1"b
?4
F
ù?9μB?￥??

T¨,?T¨<???4
Fb4
UYY4

t?4
Fí
ú?ó￡,??ó?Z
? μB?

T¨b''
LYV?èl
??
Lvl
?′8



`%}
`T¨￥_?,2TV
ü,1§9Y

Y4t?
üA9<l
?′8

`%￥}
`T¨,D

baFM1?(P<0null05),￡
L
,1§9YY

4t?9<d+s?
?f??b

(
là°ù:2010-03-02)

null179null

?26 ?2ù

2010M4

é=?D0vDD

JournalofChangchunUniversityofTraditionalChineseMedicine

Vo1null26

4,2010

?!.￥?Yb?
k?ó0a2hé?b?
k-5

?¨Morris
￡
ùT
6d,[.?ù(V?
￡?s?

ü
?
?¨
HW)?3￡  ?(V?
￡?s?ü
?￥9

??)1?
k?￥·S,
??
?ov
?4?`K

T
4Q,
?Q3￡
HW160s,?7d?:S?


k?b?
L2
?-1??
kb

1null6nullóDO?
ínullQó012ha,v
?
￡?
o

?
?J,′??
?|ó,[óN?
k b′ó;
6|

óA,3000rpm/15min ??,b

?fZE_?ób

O?
í,?ì?v
!!#
k4?
a
ü
?é??Tb

1null7nullè?nullQó012ha,
?F|v
?3o,
￡?


o?
?J,s ?O?av7?a?Z
?,?=?-?
?

%?,?!,???Z
?°·AV@,?
￡,
?,?


M?,i
è?43i
?b I3μín§M1e

%2M?b

1null8nulld9D) ?null
?μ
í
???
?[ (
?FhS

μ(nullxnulls)V
U,FW1??¨?y
íZμsb¨

SPSS13null0èqé?s,P<0null051μsμA÷?b

2null2T

2null1null ?!.ó?O?
í
￡ü￥?YnullnV1b

V1null ?!.ó?O?
í
￡ü￥?Y

FnullYó/(mmoL/L)O?
í/(U/mL)

?èF5null52null0null97nullnull30null12null5null76nullnull

?F17null15null1null55nullnull15null85null1null76nullnull

?!.F11null28null1null13#null17null73null2null54nullnull

nullnull?:D?èF1?,nullnullP<0null01;D?F1?,#P<0null05b

2null2null ?!.D:k￥?YnullnV2b

V2null ?!.D:k￥?Y

FnullY.?ù/s3￡  ?/cm

?èF17null32null5null67nullnull521null86null41null28nullnull

?F31null92null7null78nullnull845null78null45null20nullnull

?!.F22null74null6null43#null611null09null32null81nullnull

nullnull?:D?èF1?,nullnullP<0null01;D?F1?,#P<0null05b

2null3null ?!.
üí
?
￥?Ynull?F?tZ
?


üí?±2?
3n§M1M?,8D??

n,??és?aH
,éD?? ??M?,=é

??%?nM?,è0
á
6ú; ?!.5 V
ü

A???tM?(m
{)b

3null)
?

Uh7h(diabeticencephalopathy)???×
?

]?
w
ü"d??7?
3Uh?a???p?


^?×
"58??,?1VC1D:k? ?h|a

?i ?s?a
±?tas ?à ??`
±?? ??

?,K??áúw￥?
3bUh7h￥?h?


????
ü, V?D7ó5
?
a7
ü?é?Ma7

=?é
í?Matau?asè

??a7
ü%

ó§a???μ1[1-2]b

n§
^[=ú
1,?
￡=
@,á}?nD b

??aYi?9Fa????ê,=é?%á? ?

/?,L ì8?naù2?êa? y
sEaATP?

C??=??é
"?1?1?
?DVC￥
μ§

Z
T[3]bn§M1?μ%
?
aNa+-K+ATP


???p?aL ì8??p?a?a
?aá?a
μ

§
s8a3ò ó?a2?b?tì?Mo1 ó,o1

yTb??
P?
￡s=
@,?}#=?t

?n,=ìa?v
>?i??0??ì,K??á

8)3
μ§[4]b

?!μ
üA￥?aa?a???%?? F?

???r,Uhaúóaa oM?úw??%

hμ
üA
r[5-8],??n ?!]íUh7hM1

ù??b

''óù??C, ?!. VA÷??Uhv
?ó


￡ü,i
üA??a???p??
üí
?
,

4
U ?!.v
?Uh7h μ1T¨,′¤
''

?)b

参考文献:

[1]MijnhoutGS,ScheltensP,DiamantM,etal.Diabeticen-

cephalopathy:Aconceptinneedofadefinition[J].Diabetologia,

200649(6):1447-8.

[2]f￡.Uh7hù?éZ[J].
"5$Y,2003,18(8):478-479.

[3]ScottGK,AtsrikuC,KaminkerP,etal.VitaminK3(Menadione)-

InducedOncosisAssociatedwithKeratin8Phosphorylationand

HistoneH3Arylation[J].Mol.Pharmacol.,2005,68:606-615.

[4]PeiJW,TurseJE,WuQM,etal.BrucellaabortusRoughMutants

InduceMacrophageOncosisThatRequiresBacterialProteinSyn-

thesisandDirectInteractionwiththeMacrophage[J].Infect.

Immun.,2006,74:2667-2675.

[5]NambiarVS,SeshadriS.Bioavailability,trialsofbeta-carotene

fromfreshanddehydrateddrumstickleaves(Moringaoleifera)in

aratmodel[J].PlantFoodsHumNutr,2001,56(1):83-95.

[6]FrancisJA,JayaprakasamB,olsonLK,etal.InsulinSecretagogues

fromMoringaoleiferawithCyclooxygenaseEnzymeandLipid

PeroxidationInhibitoryActivities[J].Helveticachimicaacta,

2004,87(2):317-326.

[7]NdongM,UeharaM,KatsumataS,etal.EffectsoforalAdminis-

trationofMoringaoleiferaLamonGlucoseToleranceinGoto-

KakizakiandWistarRats[J].JClinBiochemNutr,2007,40

(3):229-233.

[8]GanqulyR,GuhaD.Alterationofbrainmonoamine&EEGwave

patterninratmodelofAlzheimernullsdisease&protectionby

Moringaoleifera.IndianJMedRes,2008,128(6):744-751.

(
là°ù:2010-01-09)

null180null

?26 ?2ù

2010M4

é=?D0vDD

JournalofChangchunUniversityofTraditionalChineseMedicine

Vo1null26

4,2010