SMARTersmRNA-SeqKitforIlluminaUserManual
Figure5.ExampleelectropherogramresultsforsmRNA-seqlibrariesaftersizeselectionwiththeBluePippinsystem.Librariesweregenerated
usingtheSMARTersmRNA-SeqKitforIlluminawiththeindicatedinputsandcyclingparameters,andfollowingsizeselectionwiththeBluePippin
system,wereanalyzedonanAgilent2100BioanalyzerusingtheAgilentHighSensitivityDNAKit.Peakslabeled“LM”and“UM”correspondto
DNAreferencemarkersincludedineachanalysis.Whenlibrariesareselectedusingthespecifiedrange(148–185bp),profilesexhibitanarrowpeak
extendingfrom150bpto200bp,andeitherasinglesummit(PanelA),oradoublesummit(PanelB)at~175bpduetominorvariationinsize-
selectionefficiency.
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