ToexamineifM2-EXOcouldbetakenupbyneurons,M2-EXOwereincubatedwithneuronsfor24h.3DconfocalimagesshowedthatPKH26-labeledexosomes(red)werelocalizedinthecytoplasmofMAP2+neurons(green),suggestingexosomesweretakenupbyneurons(Figure2A).
PKH26-labeledexosomesweredetectedintheischemicmousebrainafter3daysofinjection,andexosomeswerelocalizedtothecytoplasmofneurons(Figure2F).
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