智慧农业（中英文）SmartAgricultureVol.3,No.1
28
opingseeds[J].PlantMethods,2015,11(1):1-10.区域的获取[J].齐齐哈尔大学学报,2007(4):49-52.
[24]GUELPAA,DUPLESSISA,KIDDM,etal.Non-de‐ZHANGG,FENGY,LUW.Imageprocessinggray‐
structiveestimationofmaize(ZeamaysL.)kernelscaleandfeatureregionacquisition[J].JournalofQiqi‐
hardnessbymeansofanX-raymicro-computedto‐harUniversity,2007(4):49-52.
mography(μCT)densitycalibration[J].FoodandBio‐[28]GUSTINJL,JACKSONS,WILLIAMSC,etal.Anal‐
processTechnology,2015,8(7):1419-1429.ysisofmaize(Zeamays)kerneldensityandvolumeus‐
[25]GUELPAA,DUPLESSISA,MANLEYM.Ahigh-ingmicrocomputedtomographyandsingle-kernelnear-
throughputX-raymicro-computedtomography(μCT)infraredspectroscopy[J].JournalofAgriculturaland
approachformeasuringsinglekernelmaize(ZeamaysFoodChemistry,2013,61(46):10872-10880.
L.)volumesanddensities[J].JournalofCerealSci‐[29]王义发,汪黎明,沈雪芳,等.糯玉米的起源、分类、
ence,2016,69:321-328.品种改良及产业发展[J].湖南农业大学学报(自然科
[26]SHAOM,ZHANGY,DUJ,etal.Fastanalysisof学版),2007(S1):97-102.
maizekernelplumpnesscharacteristicsthroughMicro-WANGY,WANGL,SHENX.etal.Orlginclassifica‐
CTtechnology[C]//InternationalConferenceonCom‐tion,varietyimprovementandindustrydevelopmentof
puterandComputingTechnologiesinAgriculture.Ber‐waxycorn[J].JournalofHunanAgriculturalUniversity
lin,Germany:Springer,Cham,2017:31-39.(NaturalSciences),2007(S1):97-102.
[27]张桂华,冯艳波,陆卫东.图像处理的灰度化及特征
StudyontheMicro-PhenotypeofDifferentTypesofMaize
KernelsBasedonMicro-CT
1,2,31,21,21,21,2
ZHAOHuan,WANGJinglu,LIAOShengjin,ZHANGYing,LUXianju,
1,21,2
GUOXinyu,ZHAOChunjiang
（1.BeijingResearchCenterforInformationTechnology,Beijing100097,China;2.NationalEngineeringResearch
CenterforInformationTechnologyinAgriculture/BeijingKeyLabofDigitalPlant,Beijing100097,China;
3.CollegeofPlantScience&TechnologyofHuazhongAgriculturalUniversity,Wuhan430070,China）
Abstract:Plantmicro-phenotypemainlyreferstothephenotypicinformationatthetissue,cell,andsubcellularlevels,whichis
animportantpartofplantphenomicsresearch.Inviewoftheproblemsoflowefficiency,largeerror,andfewtraitsoftraditional
methodsfordetectingkernelmicroscopictraits,Micro-CTscanningtechnologywasusedtocarryoutpreciseidentificationof
micro-phenotypeon11varietiesofmaizekernels.Atotalof34microscopictraitswereobtainedbasedonCTsequenceimages
of7tissues,includingseed,embryo,endosperm,cavity,subcutaneouscavity,endospermcavityandembryocavity.Amongthe
34microscopictraits,4traits,includingendospermcavitysurfacearea,kernelvolume,endospermvolumeratioandendosperm
cavityspecificsurfacearea,weresignificantlydifferentamongmaizetypes(P-value<0.05).Thesurfaceareaofendospermcavi‐
tyandkernelvolumeofcommonmaizeweresignificantlyhigherthanthoseofothertypesofmaize.Thespecificsurfacearea
ofendospermcavityofhighoilmaizewasthelargest.Theendospermcavityofsweetcornhadthesmallestspecificsurfacear‐
ea.Theendospermvolumerationofpopcornwasthelargest.Furthermore,34traitswereusedforOne-wayANOVAandcluster
analysis,and11differentmaizevarietiesweredividedintofourcategories,ofwhichthefirstcategorywasmainlycommon
maize,thesecondcategorywasmainlypopcorn,thethirdcategorywassweetcorn,andthefourthcategorywashighoilmaize.
TheresultsindicatedthatMicro-CTscanningtechnologycouldnotonlyachievepreciseidentificationofmicro-phenotypeof
maizekernels,butalsoprovidesupportsforkernelclassificationandvarietydetection,andsoon.
Keywords:plantphenomics;micro-phenotype;Micro-CT;maizekernel;phenotypeidentification
（登陆www.smartag.net.cn免费获取电子版全文） |
|
