Vol.4,No.1张燕燕等：利用表面增强拉曼光谱定量检测植物激素脱落酸
129
QuantitativeDeterminationofPlantHormoneAbscisic
AcidUsingSurfaceEnhancedRamanSpectroscopy
1,21,21,21,21,2
ZHANGYanyan,LICan,SURui,LILinze,WEIWentao,
1,21,2,3
LIBaolei,HUJiandong
（1.CollegeofMechanicalandElectricalEngineering,HenanAgriculturalUniversity,Zhengzhou450002,China;
2.HenanInternationalJointLaboratoryofAgriculturalLaserTechnology,Zhengzhou450002,China;
3.StateKeyLaboratoryofWheatandCornCropScience,Zhengzhou450002,China）
Abstract:PlanthormoneAbscisicAcid(ABA)playsanimportantroleinregulatingplantgrowth.However,thecontentofABA
inplanttissuesisverylow,andrapidandsensitivedetectionmethodsareurgentlyneeded.Inthisstudy,arapidandquantitative
ABAdetectionmethodwasestablishedbasedonaptamerrecognitionandsurface-enhancedRamanspectroscop(SERS).The
goldnanoparticlesmodifiedbyABAaptamerhadthecharacteristicsofSERSsignalenhancementandselectiverecognition,re‐
alizingtherapidandsensitivedetectionoftraceABAincomplexplantsamplematrix.WhenABAmoleculesappearedindetect
system,theaptamerwouldspecificallybindwithABAmolecules,andtheaptamerfoldedintoG-tetradstructureatsametime,
whichwrappedABAmoleculesinthetetradstructure,shortenedthedistancebetweenABAmoleculesandgoldnanoparticles,
andtheenhancedandstableABAmoleculesSERSsignalwereobtained.Undertheconditionofoptimizedaptamerconcentra‐
tionat0.12μmol/L,differentconcentrationsofABAsolutionsinthedetectionsystemweredetected.Withintheconcentration
rangeof0.1－100μmol/L,theSERSintensityofABApresentedagoodlinearrelationshipwiththeconcentration.Thedetec‐
2
tionlimitofthismethodwas0.1μmol/LandthelinearcorrelationcoefficientRwas0.9855.Therepeatabilitytestof20points
randomlyonSERSsubstrateshowedthattherelativestandarddeviation(RSD)was6.71%,indicatingthestabilityofSERSsub‐
stratewaswell.Furthermore,thesubstrateofgoldnanoparticlesmodifiedbytheABAaptamerterminalwithsulfhydrylgroup
(SH-Apt)couldbestoredintherefrigeratorformorethanhalfayear,indicatingthatthesubstratehasgoodstability.Oncethe
preparationofthesynthesizedSH-Aptmodifiedgoldnanoparticleswascompleted.Itcouldbeusedondemandwithoutthe
needtoprepareSERSsubstrateforeverydetection.Inthissense,theconstructedaptamerSERSbiosensorcouldrealizetherap‐
idandquantitativedetectionofABA.ThemethodwasusedforthedeterminationofABAinwheatleaves,andtheresultwasin
goodagreementwiththeEnzymeLinkedImmunosorbentAssay(ELISA)(Themaxrelativeerrorwas9.13%).Thisbiosensoris
anexploratorystudyonthedetectionofplanthormonesbySERS,andtheresultsofthestudywillhaveimportantreferenceval‐
ueforthesubsequentquantitativeandon-sitedetectionofABA,aswellasthedetectionofotherplanthormones.
Keywords:abscisicacid;aptamerrecognition;surface-enhancedRamanspectroscopy;goldnanoparticles;biosensor
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