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Qual formatQual.Qual is a standard file format for read and contig quality scores.Here is an example of a qual-format read:The number of integers should be equal to the number of bases on the corresponding fasta read;qual is also the name of an input directory.It is a subdirectory of DATA and contains the raw data for... 阅78 转0 评0 公众公开 12-11-24 20:28 |
A FASTA file may contain a single or multiple sequences in FASTA format. 阅112 转0 评0 公众公开 12-11-24 20:23 |
illumina 的 mate-pair paired-end and single read以solexa为例，对单端测序(Single-read)和双端测序(Paired-end和Mate-pair)进行介绍。Paired-end方法是指在构建待测DNA文库时在两端的接头上都加上测序引物结合位点，在第一轮测序完成后，去除第一轮测序的模板链，用对读测序模块(Paired-End Module)引导互补链在原位置再生和扩增，以达到第... 阅6248 转67 评0 公众公开 12-11-18 23:01 |
mate pair and paired end used with illumina and 454 tecnologyWhat is paired-end/mate pair sequencing?For 454 technology, the terms "paired end" and "mate-pair" are used interchangeably, and the library construiction process involves circularisation and juxtaposition of the ends of long molecules, s... 阅367 转2 评0 公众公开 12-11-18 22:58 |
What is a long jumping distance (LJD) library?While the mate-pair library displays jumping distances of 2 kbp to 5 kbp, LJD libraries do show much more precise distances, thus improving assembly quality.LJD libraries are being offered for 3kbp, 8 kbp, 20 kbp and even 40 kbp jumping distance.Second, within the LJD libr... 阅280 转1 评0 公众公开 12-11-18 21:50 |
What is a mate pair library?1) Inward facing reads: Unbiotinylated fragments that have not been successfully washed away will cause undesired inward facing reads (see Illumina’s mate pair sample preparation guide). These inward-facing reads are like shotgun paired end reads as they align to the reference with a small... 阅907 转10 评0 公众公开 12-11-18 21:23 |
The differents between ends-paired and mate-paired"paired end" or "mate pair" refers to how the library is made, and then how it is sequenced.Structural rearrangements can be deduced when your read pairs map to a reference at a distance that is substantially different from how that library was cons... 阅155 转2 评0 公众公开 12-11-18 14:49 |
de bruijn and OLCHi there.In the special case of infinite coverage, if you choose the right parameters (de Bruiijn kmer=overlap=read-length-1) then the overlap and de Bruijn graphs are the same.In short - de Bruijn assemblers and overlap assemblers tend to be used on different TYPES of data with implicitly different e... 阅160 转0 评0 公众公开 12-11-11 19:47 |
摘 要: 本文阐述了生物信息学产生的背景，生物学数据库，生物信息学的主要研究内容，与生物信息学关系密切的数学和计算机科学技术领域，生物信息学产业等内容，展望了其未来并提出了若干在我国发展生物信息学的建议。生物信息学产生的背景有人说，基于序列的生物学时代已经到来，尽管对"序列生物学"这一提法可能有所争议，但是今日像... 阅663 转25 评0 公众公开 11-12-10 00:29 |
