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作者：Giriş Met al 译者：北医三院姚中强（yaozhongqiang@sina.com） 校正：孙琳 发布：柴静 审核：赵金霞 摘要： 引言:脑实质神经白塞病 (NBD) 见于5%-15% 白塞病 (BD) 患者，其特征是脑干和间脑结构的炎症。已经证实神经细胞凋亡参与了神经细胞丧失的过程。神经白塞患者血中检测出了抗神经元抗体。然而，这些抗体的致病性尚未研究过。 方法:为阐明血清抗体对神经元的潜在致病性，我们收集了7名神经白塞患者和7名健康对照的血清，并分为纯免疫球蛋白G (IgG) 和去除IgG的血清组分，而且，每一个组分都用来培养神经母细胞瘤细胞系SH-SY5Y。用毒性试验和末端转移酶介导的末端标记术(TUNEL)来评估细胞死亡。而且，使用实时定量PCR方法来评估数种凋亡相关标记物的表达。 结果:与其他处理相比，加入神经白塞患者IgG的培养的SH-SY5Y细胞死亡和凋亡均显著增加。 神经白塞患者的IgG也增加了细胞凋亡和存活通路主要分子的mRNA表达水平。 结论:我们的结果提示，来自神经白塞病患者的IgG可能通过凋亡机制而产生神经毒性。 附原文：Abstract INTRODUCTION: Parenchymal neuro-Behçet disease (NBD) isencountered in 5%-15% of Behçetdisease (BD) patients and is characterizedby inflammation of the brainstem and diencephalon structures. Neuronalapoptosis has been shown to participate in neuronal cell loss. Anti-neuronalantibodies have been identified in NBD patients. However, the pathogenicproperties of these antibodies have not been studied.METHODS:To delineate the potentialpathogenic activity of serum antibodies on neurons, pooled sera from seven NBDpatients and seven healthy controls were divided into purified immunoglobulin G(IgG) and IgG-depleted serum fractions, and each fraction was administered tocultured SH-SY5Y neuroblastoma cells. Cell death was evaluated with a toxicityassay and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)staining. Moreover, expression levels of several apoptosis markers wereevaluated with real time polymerase chain reaction (PCR).RESULTS:Administrationof NBD IgG to cultured SH-SY5Y cells induced significantly increased cell deathand apoptosis compared with other treatments. NBD IgG also enhanced the mRNAexpression levels of major apoptosis and cell survival pathway factors.CONCLUSION:Ourresults suggest that IgGs isolated from the sera of NBD patients have aneurotoxic activity that is presumably mediated by apoptotic mechanisms. 引自：Giriş M, Bireller S, Küçükali Cİ, Hanağasi H, DeğirmencioğluS, Tüzün E.Impact ofNeuro-Behçet Disease Immunoglobulin G on Neuronal Apoptosis. Noro Psikiyatr Ars. 2017 Mar;54(1):67-71.doi: 10.5152/npa.2016.19421. Epub 2016 Dec 8. |
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