GDCRNATools - An R package for downloading, organizing, and integrative analyzing lncRNA, mRNA, and miRNA data in GDC
Introduction
The Genomic Data Commons (GDC) maintains standardized
genomic, clinical, and biospecimen data from National Cancer Institute (NCI) programs including The Cancer Genome Atlas (TCGA) and Therapeutically
Applicable Research To Generate Effective Treatments (TARGET), It also accepts high quality datasets from non-NCI supported cancer research programs, such as genomic data from the Foundation
Medicine.
GDCRNATools is an R package which provides
a standard, easy-to-use and comprehensive pipeline for downloading, organizing, and integrative analyzing RNA expression data in the GDC portal with an emphasis on deciphering the lncRNA-mRNA related ceRNA regulatory network in cancer.
Many analyses can be perfomed using GDCRNATools,
including differential gene expression analysis (limma(Ritchie et al. 2015), edgeR(Robinson,
McCarthy, and Smyth 2010), and DESeq2(Love, Huber, and Anders 2014)), univariate survival
analysis (CoxPH and KM), competing endogenous RNA network analysis (hypergeometric test, Pearson correlation analysis, regulation similarity analysis, sensitivity Pearson partial correlation), and functional enrichment analysis(GO, KEGG, DO). Besides some
routine visualization methods such as volcano plot, scatter plot, and bubble plot, etc., three simple shiny apps are developed in GDCRNATools allowing users visualize the results on a local webpage.
This user-friendly package allows researchers perform the analysis by simply running a few functions and integrate their own pipelines such as molecular subtype classification, weighted
correlation network analysis (WGCNA)(Langfelder and Horvath 2008), and TF-miRNA co-regulatory network analysis, etc. into the workflow easily.
Installation
GDCRNATools is now under review in Bioconductor.
Users can install the package locally.
On
Windows system
-
Download the package GDCRNATools_0.99.0.tar.gz
-
Make sure you have Rtools installed
-
ADD R and Rtools to the Path Variable on the Environment Variables panel, including
c:\program files\Rtools\bin
c:\program files\Rtools\gcc-4.6.3\bin
c:\program files\R\R.3.x.x\bin\i386
c:\program files\R\R.3.x.x\bin\x64
-
Open a command prompt. Type R CMD INSTALL GDCRNATools_0.99.0.tar.gz
On
Linux and Mac systems
Run the following command in R
install.packages('GDCRNATools_0.99.0.tar.gz', repos = NULL, type='source')
If GDCRNATools cannot be installed due
to the lack of dependencies, please run the following code ahead to install those pacakges simutaneously or separately:
source("https:///biocLite.R")
### install packages simutaneously ###
biocLite(c('limma', 'edgeR', 'DESeq2', 'clusterProfiler', 'DOSE', 'org.Hs.eg.db', 'biomaRt', 'BiocParallel'))
install.packages(c('shiny', 'jsonlite', 'rjson', 'survival', 'survminer', 'ggplot2', 'gplots', 'Hmisc'))
### install packages seperately ###
biocLite('limma')
biocLite('edgeR')
biocLite('DESeq2')
biocLite('clusterProfiler')
biocLite('DOSE')
biocLite('org.Hs.eg.db')
biocLite('biomaRt')
biocLite('BiocParallel')
install.packages('shiny')
install.packages('jsonlite')
install.packages('rjson')
install.packages('survival')
install.packages('survminer')
install.packages('ggplot2')
install.packages('gplots')
install.packages('Hmisc')
Manual
A simply manual of GDCRNATools is available
below. Users are also highly recommended to download the comprhensive manual in .html format and view on local computer GDCRNATools
Manual
1
Data download
Two methods are provided for downloading Gene Expression Quantification (HTSeq-Counts), Isoform Expression Quantification (BCGSC miRNA Profiling), and Clinical (Clinical Supplement) data:
-
Manual download
Step1: Download GDC Data Transfer Tool on the GDC website
Step2: Add data to the GDC cart, then download manifest file and metadata of the cart
Step3: Download data using gdcRNADownload() function by providing the manifest file
-
Automatic download
Download GDC Data Transfer Tool, manifest file, and data automatically by specifying the project.id and data.type in gdcRNADownload() function
for RNAseq and miRNAs data, and in gdcClinicalDownload() function for clinical data
Users can also download data from GDC using the API method developed in TCGAbiolinks(Colaprico
et al. 2016) or using TCGA-Assembler(Zhu, Qiu, and Ji 2014)
1.1
Manual download
1.1.1 Installation of GDC Data Transfer Tool gdc-client
Download GDC Data Transfer Tool from
the GDC website and unzip the file
1.1.2 Download manifest file and metadata from GDC Data Portal
1.1.3 Download data
####### Download RNAseq data ####### gdcRNADownload(manifest = 'TCGA-PRAD/TCGA-PRAD.RNAseq.gdc_manifest.2017-11-23T14-40-52.txt', directory = 'TCGA-PRAD/RNAseq')
####### Download miRNAs data ####### gdcRNADownload(manifest = 'TCGA-PRAD/TCGA-PRAD.miRNAs.gdc_manifest.2017-11-22T15-36-57.txt', directory = 'TCGA-PRAD/miRNAs')
####### Download Clinical data ####### gdcRNADownload(manifest = 'TCGA-PRAD/TCGA-PRAD.Clinical.gdc_manifest.2017-11-23T14-42-01.txt', directory = 'TCGA-PRAD/Clinical')
1.2
Automatic download
-
gdcRNADownload() will download HTSeq-Counts data if data.type='RNAseq' and
download BCGSC miRNA Profiling data if data.type='miRNAs'. project.id argument
is required to be provided.
-
gdcClinicalDownload() download clinical data in .xml format
automatically by simply specifying the project.idargument.
1.2.1
Download RNAseq/miRNAs data
####### Download RNAseq data ####### gdcRNADownload(project.id = 'TCGA-PRAD', directory = 'TCGA-PRAD/RNAseq')
####### Download miRNAs data ####### gdcRNADownload(project.id = 'TCGA-PRAD', directory = 'TCGA-PRAD/miRNAs')
1.2.2
Download clinical data
####### Download clinical data ####### gdcClinicalDownload(project.id = 'TCGA-PRAD', directory = 'TCGA-PRAD/Clinical')
2
Data organization
2.1
Parse metadata
Metadata can be parsed by either providing the metadata file that is downloaded in the data download step, or specifying the project.id and data.type in gdcParseMetadata() function
to obtain information of data in the manifest file to facilitate data organization and basic clinical information of patients such as age, stage and gender, etc. for data analysis.
2.1.1
Parse metadata by providing the metadata file
####### Parse RNAseq metadata ####### metaMatrix.RNA <- gdcParseMetadata(metafile='TCGA-PRAD/TCGA-PRAD.RNAseq.metadata.2017-11-23T17-23-59.json')
####### Parse miRNAs metadata ####### metaMatrix.MIR <- gdcParseMetadata(metafile='TCGA-PRAD/TCGA-PRAD.miRNAs.metadata.2017-11-23T17-33-55.json')
2.1.2
Parse metadata by specifying project.id and data.type
####### Parse RNAseq metadata ####### metaMatrix.RNA <- gdcParseMetadata(project.id = 'TCGA-PRAD',
####### Parse miRNAs metadata ####### metaMatrix.MIR <- gdcParseMetadata(project.id = 'TCGA-PRAD',
2.2
Filter samples
2.2.1
Filter duplicated samples
Only one sample would be kept if the sample had been sequenced more than once by gdcFilterDuplicate().
####### Filter duplicated samples in RNAseq metadata ####### metaMatrix.RNA <- gdcFilterDuplicate(metaMatrix.RNA)
####### Filter duplicated samples in miRNAs metadata ####### metaMatrix.MIR <- gdcFilterDuplicate(metaMatrix.MIR)
2.2.2
Filter non-Primary Tumor and non-Solid Tissue Normal samples
Samples that are neither Primary Tumor (code: 01) nor Solid Tissue Normal (code: 11) would be filtered out by gdcFilterSampleType().
####### Filter non-Primary Tumor and non-Solid Tissue Normal samples in RNAseq metadata ####### metaMatrix.RNA <- gdcFilterSampleType(metaMatrix.RNA)
####### Filter non-Primary Tumor and non-Solid Tissue Normal samples in miRNAs metadata ####### metaMatrix.MIR <- gdcFilterSampleType(metaMatrix.MIR)
2.3
Merge data
-
gdcRNAMerge() merges raw counts data of RNAseq to a single expression
matrix with rows are Ensembl id and columns are samples. Total read counts for 5p and 3p strands of miRNAs can be processed from isoform quantification files and then merged to a single expression matrix with rows are miRBase v21 identifiers and
columns are samples.
-
gdcClinicalMerge() merges clinical data to a dataframe with
rows are patient id and columns are clinical traits. If key.info=TRUE, only those most commonly
used clinical traits will be reported, otherwise, all the clinical information will be reported.
2.3.1
Merge RNAseq/miRNAs data
####### Merge RNAseq data ####### rnaMatrix <- gdcRNAMerge(metadata = metaMatrix.RNA, path = 'TCGA-PRAD/RNAseq/',
####### Merge miRNAs data ####### mirMatrix <- gdcRNAMerge(metadata = metaMatrix.MIR, path = 'TCGA-PRAD/miRNAs/',
2.3.2
Merge clinical data
####### Merge clinical data ####### clinicalDa <- gdcClinicalMerge(path = 'TCGA-PRAD/Clinical/', key.info = TRUE)
2.4
TMM normalization and voom transformation
It has repeatedly shown that normalization is a critical way to ensure accurate estimation and detection of differential expression (DE) by removing systematic technical effects that occur
in the data(Robinson and Oshlack 2010). TMM normalization is a simple and effective method for estimating relative RNA production levels from RNA-seq data. Voom is moreover faster and more convenient than existing RNA-seq methods, and converts RNA-seq data
into a form that can be analyzed using similar tools as for microarrays(Law et al. 2014).
By running gdcVoomNormalization() function,
raw counts data would be normalized by TMM method implemented in edgeR(Robinson, McCarthy,
and Smyth 2010) and further transformed by the voom method provided in limma(Ritchie et al.
2015). Low expression genes (logcpm < 1 in more than half of the samples) will be filtered out by default. All the genes can be kept by setting filter=TRUE in
the gdcVoomNormalization().
####### RNAseq data ####### rnaExpr <- gdcVoomNormalization(counts = rnaMatrix, filter = FALSE)
####### miRNAs data ####### mirExpr <- gdcVoomNormalization(counts = mirMatrix, filter = FALSE)
3.
Differential gene expression analysis
gdcDEAnalysis(), a convenience wrapper,
provides three widely used methods limma(Ritchie et al. 2015), edgeR(Robinson,
McCarthy, and Smyth 2010), and DESeq2(Love, Huber, and Anders 2014) to identify differentially
expressed genes (DEGs) or miRNAs between any two groups defined by users. Note that DESeq2(Love,
Huber, and Anders 2014) maybe slow with a single core. Multiple cores can be specified with the nCore argument
if DESeq2(Love, Huber, and Anders 2014) is in use. Users are encouraged to consult the vignette
of each method for more detailed information.
3.1
DE analysis
DEGAll <- gdcDEAnalysis(counts = rnaMatrix, group = metaMatrix.RNA$sample_type, comparison = 'PrimaryTumor-SolidTissueNormal',
3.2
Report DE genes/miRNAs
All DEGs, DE long non-coding genes, DE protein coding genes and DE miRNAs could be reported separately by setting geneType argument
in gdcDEReport(). Gene symbols and biotypes based on the Ensembl 90 annotation are reported in the output.
deALL <- gdcDEReport(deg = DEGAll, gene.type = 'all')
deLNC <- gdcDEReport(deg = DEGAll, gene.type = 'long_non_coding')
#### DE protein coding genes dePC <- gdcDEReport(deg = DEGAll, gene.type = 'protein_coding')
3.3
DEG visualization
Volcano plot and bar plot are used to visualize DE analysis results in different manners by gdcVolcanoPlot() and gdcBarPlot() functions,
respectively . Hierarchical clustering on the expression matrix of DEGs can be analyzed and plotted by the gdcHeatmap() function.
3.3.1
Volcano plot
gdcVolcanoPlot(DEGAll)
3.3.2
Barplot
gdcBarPlot(deg = deALL, angle = 45, data.type = 'RNAseq')
3.3.3
Heatmap
Heatmap is generated based on the heatmap.2() function
in gplots package.
degName = rownames(deALL)
gdcHeatmap(deg.id = degName, metadata = metaMatrix.RNA, rna.expr = rnaExpr)
4
Competing endogenous RNAs network analysis
Three criteria are used to determine the competing endogenous interactions between lncRNA-mRNA pairs:
- The lncRNA and mRNA must share significant number of miRNAs
- Expression of lncRNA and mRNA must be positively correlated
- Those common miRNAs should play similar roles in regulating the expression of lncRNA and mRNA
4.1
Hypergeometric test
Hypergenometric test is performed to test whether a lncRNA and mRNA share many miRNAs significantly.
A newly developed algorithm spongeScan(Furi’o-Tar’i
et al. 2016) is used to predict MREs in lncRNAs acting as ceRNAs. Databases such as starBase v2.0(J.-H.
Li et al. 2014), miRcode(Jeggari, Marks, and Larsson 2012) and mirTarBase
release 7.0(Chou et al. 2017) are used to collect predicted and experimentally validated miRNA-mRNA and/or miRNA-lncRNA interactions. Gene IDs in these databases are updated to the latest Ensembl 90 annotation of human genome and miRNAs names are
updated to the new release miRBase 21 identifiers. Users can also provide their own datasets of miRNA-lncRNA and miRNA-mRNA interactions.
The figure and equation below illustrate how the hypergeometric test works
here  is
the number of shared miRNAs,  is
the total number of miRNAs in the database,  is
the number of miRNAs targeting the lncRNA,  is
the number of miRNAs targeting the protein coding gene.
4.2
Pearson correlation analysis
Pearson correlation coefficient is a measure of the strength of a linear association between two variables. As we all know, miRNAs are negative regulators of gene expression. If more common
miRNAs are occupied by a lncRNA, less of them will bind to the target mRNA, thus increasing the expression level of mRNA. So expression of the lncRNA and mRNA in a ceRNA pair should be positively correlated.
4.3
Regulation pattern analysis
Two methods are used to measure the regulatory role of miRNAs on the lncRNA and mRNA:
We defined a measurement regulation similarity score to check the similarity between miRNAs-lncRNA expression correlation and miRNAs-mRNA expression correlation.
where is
the total number of shared miRNAs, is
the th
shared miRNAs, and represents
the Pearson correlation between the th
miRNA and lncRNA, the th
miRNA and mRNA, respectively
Sensitivity correlation is defined by Paci et al.(2014) to measure if the correlation between a lncRNA and mRNA is mediated by a miRNA in the lncRNA-miRNA-mRNA triplet. We take average of
all triplets of a lncRNA-mRNA pair and their shared miRNAs as the sensitivity correlation between a selected lncRNA and mRNA.
where is
the total number of shared miRNAs, is
the th
shared miRNAs, , and represents
the Pearson correlation between the long non-coding RNA and the protein coding gene, the kth miRNA and lncRNA, the kth miRNA and mRNA, respectively
The hypergeometric test of shared miRNAs, expression correlation analysis of lncRNA-mRNA pair, and regulation pattern analysis of shared miRNAs are all implemented in the gdcCEAnalysis() function.
ceOutput <- gdcCEAnalysis(lnc = rownames(deLNC), lnc.targets = 'starBase',
4.4
ceRNAs visualization
4.4.1
Correlation plot
gdcCorPlot(gene1 = 'ENSG00000234456', gene2 = 'ENSG00000105971', metadata = metaMatrix.RNA)
4.4.2
Correlation plot on a local webpage by shinyCorplot
Typing and running gdcCorPlot() for each
pair of lncRNA-mRNA is bothering when multiple pairs are being interested in. shinyCorPlot() , a interactive
plot function based on shiny package, can be easily operated by just clicking the genes in each drop down
box (in the GUI window). By running shinyCorPlot() function, a local webpage would pop up and correlation
plot between a lncRNA and mRNA would be automatically shown.
shinyCorPlot(gene1 = rownames(deLNC), metadata = metaMatrix.RNA)
4.4.3
Network visulization in Cytoscape
lncRNA-miRNA-mRNA interactions can be reported by the gdcExportNetwork() and
visualized in Cytoscape.
ceOutput2 <- ceOutput[ceOutput$hyperPValue<0.01 & ceOutput$corPValue<0.01 & ceOutput$regSim != 0,]
edges <- gdcExportNetwork(ceNetwork = ceOutput2, net = 'edges') nodes <- gdcExportNetwork(ceNetwork = ceOutput2, net = 'nodes')
5
Univariate survival analysis
Two methods are provided to perform univariate survival analysis: Cox Proportional-Hazards (CoxPH) model and Kaplan Meier (KM) analysis based on the survival package.
CoxPH model considers expression value as continous variable while KM analysis divides patients into high-expreesion and low-expression groups by a user-defined threshold such as median or mean. gdcSurvivalAnalysis() take
a list of genes as input and report the hazard ratio, 95% confidence intervals, and test significance of each gene on overall survival.
5.1
CoxPH analysis
####### CoxPH analysis ####### survOutput <- gdcSurvivalAnalysis(gene = rownames(deALL), metadata = metaMatrix.RNA)
5.2
KM analysis
####### KM analysis ####### survOutput <- gdcSurvivalAnalysis(gene = rownames(deALL), metadata = metaMatrix.RNA,
5.3
KM analysis visualization
5.5.1
KM plot
KM survival curves are ploted using the gdcKMPlot() function
which is based on the R package survminer.
gdcKMPlot(gene = 'ENSG00000197275', metadata = metaMatrix.RNA,
5.3.2
KM plot on a local webpage by shinyKMPlot
The shinyKMPlot() function is also a simply shiny app
which allow users view KM plots of all genes of interests on a local webpackage conveniently.
shinyKMPlot(gene = rownames(deALL), rna.expr = rnaExpr, metadata = metaMatrix.RNA)
6
Functional enrichment analysis
One of the main uses of the GO is to perform enrichment analysis on gene sets. For example, given a set of genes that are up-regulated under certain conditions, an enrichment analysis will
find which GO terms are over-represented (or under-represented) using annotations for that gene set and pathway enrichment can also be applied afterwards.
6.1
GO, KEGG and DO analyses
gdcEnrichAnalysis() can perform Gene ontology
(GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and Disease Ontology (DO) functional enrichment analyses of a list of genes simultaneously. GO and KEGG analyses are based on the R/Bioconductor packages clusterProfilier(Yu
et al. 2012) and DOSE(Yu et al. 2015). Redundant GO terms can be removed by specifying simplify=TRUE in
the gdcEnrichAnalysis() function which uses the simplify() function
in the clusterProfilier(Yu et al. 2012) package.
enrichOutput <- gdcEnrichAnalysis(gene = rownames(deALL), simplify = TRUE)
6.2
Enrichment visualization
The output generated by gdcEnrichAnalysis() can
be used for visualization in the gdcEnrichPlot() function by specifying type,category and numTerms arguments.
6.2.1
GO barplot
gdcEnrichPlot(enrichOutput, type = 'bar', category = 'GO', num.terms = 10)
6.2.2
GO bubble plot
gdcEnrichPlot(enrichOutput, type='bubble', category='GO', num.terms = 10)
6.2.3
KEGG/DO barplot
gdcEnrichPlot(enrichment = enrichOutput, bar.color = 'chocolate1',
gdcEnrichPlot(enrichment = enrichOutput, bar.color = 'dodgerblue',
6.2.4
KEGG/DO bubble plot
gdcEnrichPlot(enrichOutput, category='KEGG',type = 'bubble', num.terms = 20)
gdcEnrichPlot(enrichOutput, category='DO',type = 'bubble', num.terms = 20)
6.2.5
Pathview
Users can visualize a pathway map with pathview() function
in the pathview(Luo and Brouwer 2013) package. It displays related many-genes-to-many-terms
on 2-D view, shows by genes on BioCarta & KEGG pathway maps. Gradient colors can be used to indicate if genes are up-regulated or down-regulated.
names(deg) <- rownames(deALL)
hsa04022 <- pathview(gene.data = deg, limit = list(gene=max(abs(geneList)), cpd=1))
6.2.6
View pathway maps on a local webpage by shinyPathview
shinyPathview() allows users view and
download pathways of interests by simply selecting the pathway terms on a local webpage.
pathways <- as.character(enrichOutput$Terms[enrichOutput$Category=='KEGG'])
shinyPathview(deg, pathways = pathways, directory = 'pathview')
sessionInfo
sessionInfo()
References
Chou, Chih-Hung, Sirjana Shrestha, Chi-Dung Yang, Nai-Wen Chang, Yu-Ling Lin, Kuang-Wen Liao, Wei-Chi Huang, et al. 2017. “MiRTarBase Update 2018: A Resource for Experimentally Validated MicroRNA-Target
Interactions.” Nucleic Acids Research, November, gkx1067–gkx1067. doi:10.1093/nar/gkx1067.
Colaprico, Antonio, Tiago C. Silva, Catharina Olsen, Luciano Garofano, Claudia Cava, Davide Garolini, Thais S. Sabedot, et al. 2016. “TCGAbiolinks: An R/Bioconductor Package for Integrative
Analysis of TCGA Data.” Nucleic Acids Research 44 (8): e71. doi:10.1093/nar/gkv1507.
Furi’o-Tar’i, Pedro, Sonia Tarazona, Toni Gabald’on, Anton J. Enright, and Ana Conesa. 2016. “SpongeScan: A Web for Detecting MicroRNA Binding Elements in LncRNA Sequences.” Nucleic Acids
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