穴位局部外泌体参与针刺镇痛效应启动的研究

369蓝田书院 2019-08-28

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Participation of local exosomes of acupoints in the initiation of acupuncture analgesic effect

穴位局部外泌体参与针刺镇痛效应启动的研究

Bo CHEN (陈波)1,2 Mu-yang LI (李牧洋)1, Li-ying XING (邢立莹)1, Xue-mao ZHUO (卓雪茂)1,Ze-lin CHEN (陈泽林)1,2,Yi GUO (郭义)1,3, Ming-yue LI (李明月)2 *

a Experimental Acupuncture and Moxibustion Research Center, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China(天津中医药大学实验针灸学研究中心,天津 300193，中国)

b College of Acupuncture and Massage, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China (天津中医药大学针灸推拿学院,天津 300193，中国)

c Chinese Medical College of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China(天津中医药大学中医学院,天津 300193，中国)

☆Supported by Scientific Research Project of Tianjin Municipal Education Commission: 2017KJ143; National Natural Science Foundation of China: NO.81403457, NO. 81330088

*Corresponding author.

E-mail address: limingyue7@163.com(M-y.LI )

ABSTRACT

Objective: To investigate the local initiation role of local exosomes of acupoints in acupuncture analgesic effect. Methods: Thirty-two rats with adjuvant arthritis were randomly divided into model group (Group CFA), model + electroacupuncture group (Group EA), model + antagonist + electroacupuncture group (Group GW4869+EA), and model + dimethyl sulfoxide + electroacupuncture group (Group DMSO+EA), with 8 rats in each group. The model rat s of adjuvant arthritis were prepared by intradermal injection of 0.1 mL of Freund's adjuvant complete into the metatarsal of the right posterior foot to induce inflammation. No intervention was given in Group CFA, while electroacupuncture was performed in the other three groups at “Zúsānlĭ (足三里ST 36, bilaterally)” and “Huántiào (环跳GB 30, bilaterally)” of the rats with the following electroacupuncture parameters: dilatational wave with a frequency of 2/10 Hz, an intensity of 5/10/15 (0.76/1.53/2.3mA), a duration of 30 min, and an intensity increasing every 10 min. In Group DMSO+EA, DMSO (with a concentration of 0.2%, 50μL/acupoint) was injected at bilateral “ST 36” of the model rats one hour before electroacupuncture, while GW4869 (with a concentration of 3 mg/mL, 50μL/acupoint) was injected at bilateral ST 36 of the model rats one hour before electroacupuncture in Group GW4869+EA. The paw withdrawal latency (PWL) was used as the therapeutic effect index.

Results: The PWL of rats in each group at Hour 24 after modeling was significantly lower than that before modeling, indicating that the models were successfully established. After the electroacupuncture treatment, the PWL of rats showed an increasing trend in all groups. The PWL of Group GW4869+EA (6.74±1.09) s was lower than that of Group EA (7.72±1.54) s on Day 1 after injection, but the difference was not statistically significant (P>0.05). The PWL values of Group GW4869+EA (7.72±0.70) s, (7.87±0.58) s were significantly lower than those of Group EA (9.96±0.94)s, (9.66±0.96) s (both P < 0.05) on Days 2 & 3 after injection, and the differences were statistically significant.

Conclusion: It was preliminarily found that acupuncture analgesic effect was significantly reduced after local exosomes of acupoints were blocked, indicating that local exosomes of acupoints may be involved in the initiation process of acupuncture effect.

Key words:

Acupoint；acupuncture analgesia；Exosome；antagonism

ABSTRACT IN CHINESE

[摘要] 目的：探讨穴位局部外泌体（exosome）在针刺镇痛效应起效过程中的穴位局部启动作用。方法：将32只佐剂性关节炎（RA）大鼠随机分为模型组（CFA组）、模型+电针组（EA组），模型+拮抗剂+电针组（GW4869+EA组），模型+二甲基亚砜+电针组（DMSO+EA组）。大鼠右后足跖皮内注射弗氏完全佐剂0.1 mL致炎,制备佐剂性关节炎动物模型。其中模型组不予干预，其他三组电针针刺大鼠双侧“足三里”“ 环跳”穴，电针参数疏密波，频率2/10 Hz，强度5/10/15（0.76/1.53/2.3 mA），持续时间30 min，每隔10 min增加强度。其中DMSO+EA组电针针刺前1小时，于模型大鼠双侧“足三里”穴给予DMSO（浓度为0.2%，50μL/穴）穴位注射；GW4869+EA组电针针刺前1小时，于模型大鼠双侧“足三里”穴给予GW4869（浓度为3mg/mL，50μL/穴）穴位注射，以热辐射缩足痛阈（paw withdrawal latency，PWL）为疗效指标。结果：造模后24小时,各组大鼠热辐射缩足痛阈较造模前均明显降低，提示造模成功。电针治疗后，各组大鼠热辐射缩足痛阈均呈现上升趋势，注射第1天GW4869+EA组大鼠热辐射缩足痛阈（6.74±1.09）s较EA组（7.72±1.54）s低，但差异无统计学意义（P>0.05）；注射第2、3天GW4869+EA组大鼠热辐射缩足痛阈（7.72±0.70）s、(7.87±0.58）s较EA组（9.96±0.94）s、(9.66±0.96）s显著降低（均P﹤0.05），差异存在统计学意义。结论：初步发现阻断穴位局部外泌体后针刺镇痛效应显著减弱，说明穴位局部外泌体可能参与了针刺穴位效应启动过程。

［关键词］穴位；针刺镇痛；外泌体；拮抗

《世界针灸杂志》World Journal of Acupuncture-Moxibustion

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　　2012年，《世界针灸杂志》被收录进入Science Direct数据库。2015年，被中国科学引文数据库（CSCD）核心库收录。2016年被国际著名科学文献出版公司科睿唯安（原汤森路透集团） Emerging Sources Citation Index（ESCI）收录，可在Web of Science 核心集合子集中查询，与SCI源刊同路径检索与下载。《世界针灸杂志》编委会由全球范围内的46位知名专家组成，其中境外专家占58.7%。2018年起，《世界针灸杂志》成为爱思唯尔全流程合作期刊，全面实现了出版流程和排版的国际化。

　　World Journal of Acupuncture-Moxibustion (WJAM) was established in 1991, which has been administered by State Administration of Traditional Chinese Medicine of the People's Republic of China, sponsored by World Federation of Acupuncture-Moxibustion Societies (WFAS), Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences (CACMS) and China Association of Acupuncture and Moxibustion (CAAM). It is an English version academic journal from China and publish throughout the world, and the Italian version is published correspondingly.

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