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植物分期的小干扰RNA（phasiRNA）有助于雄性强健的生育能力。 但是，特定功能仍未定义。 在玉米（Zea mays）中，雄性不育23（ms23）是24-nt phasiRNA前体（24-PHAS）基因座和Dicer-like5（Dcl5）表达所必需的，而无法切割PHAS转录本的dcl5-1-1突变体几乎没有24 -nt phasiRNA。 基于序列捕获亚硫酸氢盐测序，我们发现大多数24-PHAS位点的CHH DNA甲基化在对照植物的减数分裂花药中增加，但在ms23和dcl5突变体中没有增加。 因为dcl5-1花药表达PHAS前体， 所以我们得出结论，在这些位点靶向增加CHH甲基化需要24-nt phasiRNA，而不仅仅是激活PHAS转录。 尽管PHAS前体被加工成多种24-nt phasiRNA产物，但仍有大量差异产物积聚。 丰富的24-nt phasiRNA位置对应于单个基因座中的高CHH甲基化，进一步证明了24-nt phasiRNA促进顺式CHH甲基化的结论。 Plant phased small interfering RNAs (phasiRNAs) contribute to robust male fertility; however, specific functions remain undefined. In maize (Zea mays), male sterile23 (ms23), necessary for both 24‐nt phasiRNA precursor (24‐PHAS) loci and Dicer‐like5 (Dcl5) expression, and dcl5‐1 mutants unable to slice PHAS transcripts lack nearly all 24‐nt phasiRNAs. Based on sequence capture bisulfite‐sequencing, we find that CHH DNA methylation of most 24‐PHAS loci is increased in meiotic anthers of control plants but not in the ms23 and dcl5 mutants. Because dcl5‐1 anthers express PHAS precursors, we conclude that the 24‐nt phasiRNAs, rather than just activation of PHAS transcription, are required for targeting increased CHH methylation at these loci. Although PHAS precursors are processed into multiple 24‐nt phasiRNA products, there is substantial differential product accumulation. Abundant 24‐nt phasiRNA positions corresponded to high CHH methylation within individual loci, reinforcing the conclusion that 24‐nt phasiRNAs contribute to increased CHH methylation in cis.  文章原创，如有侵权请及时联系PaperRSS小编删除，转载请注明来源。 温馨提示： 为方便PaperRSS粉丝们科研、就业等话题交流。我们根据10多个专业方向（植物、医学、药学、人工智能、化学、物理、财经管理、体育等），特建立了30个国内外博士交流群。群成员来源欧美、日韩、新加坡、清华北大、中科院等全球名校。 |
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