Hepatic CD4+ or CD8+ T-cells of 12 months NASH-diet + 8 weeks treatment (IgG, α-PD-1 or α-CD8) mice were isolated by liver perfusion, mechanical + enzymatic digestion and percoll gradient enrichment and subsequently single-cell sorted (sorted gate: L/D-,CD11b-,CD11c-,CD19-,CD45+,CD4+ or CD8+ cells) and frozen on dry-ice .
We isolated liver sinusoidal endothelial cells (LSEC) from mice fed control chow (Chow) or the nonalcoholic steatohepatitis (NASH)-inducing high fat, fructose and cholesterol (FFC) diet, and subjected the cells to bulk RNA-seq.
Out of 13993 transcripts detected, the adhesion molecule Vcam1 was significantly upregulated in LSEC from FFC-fed mice compared to Chow-fed mice (Log2FC: 0.925, p: 3.6x10E-18), whereas the upregulation of another adhesion molecule Icam1 (Log2FC: 0.416, p: 0.014) was not as striking as Vcam1.