文章题目:Cross-tissue organization of the fibroblast lineage 发表在:Nature 日期:12 May 2021 链接:https://www./articles/s41586-021-03549-5 作者:Genentech团队
基因泰克公司(英语:Genentech Inc.),全名基因工程科技公司(英语:Genetic Engineering Technology),是一家生物科技公司,由创投人罗伯特·史旺森(Robert A. Swanson)与生物化学家赫伯特·博耶(Herbert Boyer)博士于1976年成立,这件事也被视为是生物科技产业的起点。罗氏药厂于2009年3月26日以大约468亿美元完成了基因泰克的收购,并完全拥有此公司。截至2019年2月为止,基因泰克有超过13,697名员工。
还发了一个在线工具:https://www./ FibroXplorer is an interactive data portal that allows users to examine gene expression at the single-cell level in ~230,000 mouse fibroblasts from 17 tissues, 50 datasets and 12 disease states
Cxcl12+ mesenchymal stromal cells and osteolineage cells,
Fbln1+ and Bmp4+ intestinal fibroblasts,
Comp+ fibroblasts,
Npnt+ alveolar fibroblasts
Hhip+ peribronchial fibroblasts
几乎所有的组织都有Pi16+ and Col15a1+ clusters
DEGs in the Pi16+ cluster (Pi16, Dpp4 and Ly6c1) suggested an identity similar to adventitial stromal cells Col15a1+ cluster exhibited an association with the basement membrane, evidenced by expression of Col4a1, Hspg2 and Col15a1
图d是:Slingshot lineage inference identified trajectories that emerged from the Pi16+ cluster, passed through the Col15a1+ cluster, and ended at specialized clusters
接着,拿C3 PDAC CAFs + colon fibroblasts + lung fibroblasts from individuals with non-small cell lung cancer (NSCLC), IPF or COVID-19 (n = 10,355 cells)进行整合,得到6个cluster
NPNT+ alveolar fibroblasts derived from individuals with lung disease
ADAMDEC1+ and CCL19+31 clusters primarily from colitis samples
PI16+ cluster, in which the human universal signature was most enriched
two myofibroblast clusters defined by LRRC15+ and COL3A1+ expression:
LRRC15+ myofibroblasts were enriched in cells from individuals with pancreatic and lung cancer,
COL3A1+ cluster was enriched in cells from patients with COVID-19
进而验证了小鼠的图谱:LRRC15+ myofibroblasts and ADAMDEC1+ fibroblasts
另外还发现了COVID-19病人中存在一个独特的myofibroblast细胞群
最后留下疑问:It remains unclear why two universal Dpt+ fibroblast subtypes exist, though we speculate that this may represent a necessary division of labour within the lineage.