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脱落酸（ABA）是介导植物适应各种胁迫的许多信号网络的关键组成部分。在这种情况下，ABA诱导的抗氧化防御被认为是提高植物耐水性的主要机制。然而，这种激活的具体细节仍然难以捉摸。在这项工作中，我们发现来自新R3H家族的蛋白质DIP1在调节水稻的耐水性方面起着核心作用。OsDIP1转录本由过氧化氢（H2O2）、ABA、干旱（聚乙二醇处理）和盐胁迫诱导。OsDIP1在水稻中的过度表达增强了水稻的耐旱性和耐盐性，而通过CRISPR-Cas9编辑敲除OsDIP1则导致干旱和盐敏感表型。在OsDIP1过表达的转基因水稻植株中，抗氧化酶、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）的活性和基因表达增加，而丙二醛（MDA）含量降低。 相反，敲除OsDIP1的水稻植株中SOD和CAT的含量和基因表达降低，MDA含量增加，这表明OsDIP1的过度表达增强了水稻植株的抗氧化能力。酵母双杂交筛选试验表明OsDIP1与ZFP36相互作用，ZFP36是参与ABA诱导抗氧化防御的关键锌指转录因子。此外，OsDIP1可以通过与ZFP36相互作用来调节一些关键的ABA应答基因。总的来说，我们的研究结果表明OsDIP1在ABA诱导的水稻抗氧化防御信号和适应盐分和干旱中起着重要作用。 Abscisic acid (ABA) is a key component of many signaling networks mediating plant adaptation to various stresses. In this context, ABA-induced antioxidant defence is considered to be a main mechanism to that enhances water stress tolerance in plants. The specific details of this activation remain, however, elusive. In this work, we show that DIP1, a protein from novel R3H family, played a central role in modulating water stress tolerance in rice. OsDIP1 transcripts were induced by hydrogen peroxide (H2O2), ABA, drought (polyethylene glycol treatment), and salt stress. Overexpression of OsDIP1 in rice enhanced drought and salinity tolerance while knocking out OsDIP1 by CRISPR-Cas9 editing resulted in drought and salt sensitive phenotype. The activity and gene expression of antioxidant defence enzymes, superoxide dismutase (SOD), catalase (CAT), increased in OsDIP1-overexpressed transgenic rice plants, while the content of malondialdehyde (MDA) decreased. In contrast, the content and gene expression of SOD and CAT, decreased, and the content of MDA increased in knockout of OsDIP1 rice plants, suggesting that overexpression of OsDIP1 enhances the antioxidant capacity of rice plants. The yeast two hybrid screening test revealed that OsDIP1 interacted with ZFP36, a key zinc finger transcription factor involved in ABA-induced antioxidant defence. Moreover, OsDIP1 could modulate some key ABA-responsive genes via interacting with ZFP36. Overall, our findings indicate an important role of OsDIP1 in ABA-induced antioxidant defence signaling and adaptation to salinity and drought in rice.  文章来源网络整理；如有侵权请及时联系PaperRSS小编删除，转载请注明来源。 |
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