【原】哺乳动物翻译起始因子可以在体内替代其酵母同系物

A mammalian translation initiation factor can substitute for its yeast homologue in vivo.

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|核心内容：

翻译起始因子4E(EIF-4E)参与真核细胞mRNA 5'端帽子结构的识别，促进核糖体结合。

随后，额外的启动因子介导了mRNA的核糖体扫描和启动子Aug的识别(Shatkin，A.J.(1985)Cell 40,223-224；Rhoads，R.E.(1988)Trends Biochem)。SCI。13，52-56；Edery，I.，Pelletier，J.和Sonenberg，N.(1987)，《基因表达的翻译调控》(Ilan，J.，编辑)第335-366页，Plenum Publishing Corp.，纽约)。

在这里，我们发现启动因子4E在单细胞真核生物酿酒酵母和哺乳动物之间功能上是保守的。

虽然这两种因子的氨基酸同源性仅为33%，但小鼠eIF-4E可以在体内替代酵母eIF-4E，而不会对细胞活力、生长和交配产生重大影响。

这一发现为研究真核细胞翻译起始因子eIF-4E的结构-功能关系提供了新的实验策略。

原文摘要：

The translation initiation factor 4E (eIF-4E) is involved in the recognition of the cap structure at the 5' end of eukaryotic mRNA and facilitates ribosome binding. Subsequently, additional initiation factors mediate ribosomal scanning of mRNA and initiator AUG recognition (Shatkin, A. J. (1985) Cell 40, 223-224; Rhoads, R. E. (1988) Trends Biochem. Sci. 13, 52-56; Edery, I., Pelletier, J., and Sonenberg, N. (1987) in Translational Regulation of Gene Expression (Ilan, J., ed) pp. 335-366, Plenum Publishing Corp., New York). We show here that initiation factor 4E is functionally conserved between the unicellular eukaryote Saccharomyces cerevisiae and mammals. Although the amino acid identity of the factors from both species is limited to only 33%, mouse eIF-4E can substitute for yeast eIF-4E in vivo without major effects on cell viability, growth, and mating. This finding provides a starting point for new experimental strategies to investigate the structure-function relationship of eukaryotic translation initiation factor eIF-4E.

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参考文献：https://www./article/S0021-9258(18)63833-5/pdf

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