【原】胚胎干细胞标记物存在于兔动脉粥样硬化斑块中

Embryonic stem cells markers are present within rabbit atherosclerotic plaques.

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|核心内容：

未标记：最近的证据表明动脉粥样硬化斑块中的平滑肌细胞来源于血管前体细胞。

我们以前已经证明，兔动脉粥样硬化斑块中的平滑肌细胞和巨噬细胞对肾素、血管紧张素和一氧化氮系统的因子以及造血干细胞标志物CD34和泛白细胞标志物CD45呈阳性反应。

为了探讨这些细胞是原始类型的观点，用免疫组织化学方法鉴定了这些斑块中的多能胚胎干细胞(ESC)标记物(OCT-4、SSEA1、3、4、TRA1-60、81)，并将其与内膜增厚进行了比较。

目的：对ESC标志物在兔主动脉内膜增厚和动脉粥样硬化斑块中的表达进行免疫定位。

设计：新西兰大白兔饲喂对照日粮(CON)、0.5%胆固醇(Chol)或1%蛋氨酸(Meth)，饲养12周。动物灌流固定，主动脉切除，石蜡切片。免疫组织化学采用标准技术。

结果：OCT-4、SSEA 1、3和4、TRA-1-60和TRA-1-81均存在于动脉粥样硬化斑块内。但部分细胞TRA-1-60和TRA-1-81不表达。

事实上，TRA-1-81阳性的巨噬细胞很少见，TRA-1-81阳性的平滑肌细胞也很少见。Meth内膜增厚未见TRA-1-81阳性细胞。

结论：动脉粥样硬化斑块内巨噬细胞和平滑肌细胞表达ESC标志物。这些结果表明，这些斑块内的细胞是原始的，并可能分化为其他类型的细胞。

原文摘要：

UNLABELLED:Recent evidence suggests that smooth muscle cells within atherosclerotic plaques originate from vascular progenitor cells. We have previously shown that smooth muscle cells and macrophages present within rabbit atherosclerotic plaques are positive for factors of the renin angiotensin and nitric oxide systems as well as the hematopoietic stem-cell marker CD34 and the pan-leukocyte marker CD45. To explore the idea that these cells are of primitive types, immunohistochemistry was used to identify pluripotent embryonic stem cells (ESC) markers (Oct-4, SSEA1,3,4, TRA1-60, 81) in these plaques and to compare these to intimal thickening. OBJECTIVE:To immunolocalise ESC markers in rabbit aortic intimal thickening and atherosclerotic plaques. DESIGN:New Zealand White rabbits were fed either a control (Con) diet, 0.5% cholesterol (Chol) or 1% methionine (Meth) for 12 weeks. Animals were perfusion fixed, aortae excised and processed for paraffin. Immunohistochemistry was performed by standard techniques. RESULTS:Oct-4, SSEA 1, 3 and 4, TRA-1-60 and TRA-1-81 were all present within in atherosclerotic plaques. However, some cells were not positive for TRA-1-60 and TRA-1-81. In fact, positive TRA-1-81 macrophages were uncommon, and positive TRA-1-81 smooth muscle cells were rare. Intimal thickening in Meth did not show any TRA-1-81 positive cells CONCLUSIONS:Macrophages and smooth muscle cells within atherosclerotic plaques express markers of ESC. These results suggest that cells within these plaques are primitive and might differentiate into other types of cells.

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参考文献：10.14670/HH-23.741

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