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SnapGene通俗说就是好看又好用!它可以用来查看和编辑质粒图谱(酶切位点、基因片段属性、引物、开放阅读框所编码氨基酸、多序列比对、自动引物设计等等)。当然,除质粒外,DNA序列甚至基因组都可以用SnapGene来查看和操作。更多功能请下载安装后研读下文“使用指南(常用功能示范)”。
资料领取:关注本公众号,后台发送 “SG” 已关注的读者可以下拉到本文底部直接发 “SG”,其实发送小写“sg”也可以的,系统不区分大小写。 :为了完整地展示软件安装过程,我把我原来安装的软件uninstall了。用心良苦! 第一步:
第二步: 选择安装位置--NEXT--I Agree--勾选“Desktop Icon”(在桌面显示软件快捷方式)--NEXT--Install。 第三步:点击finish(不要急着勾选"Run SnapGene",这一步很关键,我单独列出来,心急吃不了热豆腐啊)。
还记得刚刚说到有个“Crack”文件吗?绿色框框里的,现在需要它了。 1. 打开Crack文件夹,同时复制ActivationCrack和SnapGene文件。 2. 打开你安装的SnapGene文件夹,鼠标放到空白处点击右键并粘贴,替换原来同名文件夹。 3. 双击ActivationCrack.exe文件后点击PatchAndRegister,然后会有询问消息,因为这个补丁本来是用来解3.2.1的,现在安装的版本是4.,但是也能破,所以点击Yes;系统提示”Patch and Activation successful!! Enjoy.“就表示成功了。 如何使用?
文末加点料: 推荐一个质粒信息非常全面也非常实用的网站http://www./ ---分割线--- "I just want to say that I LOVE SnapGene. It has dramatically altered the research that my lab does, giving us the confidence to take on difficult cloning with minimal effort! "-----D. Bryant University of Glasgow “SnapGene is the most intuitive, easy to use cloning program I have ever been able to get my hands on. ”----D. Kraut Villanova University [ɪnˈtuːɪtɪv] 直观的;凭直觉得到的;直觉的;有直觉力的;易懂的;使用简便的 【in 不 + tuit 保护;指导,教育 + ive 有…性质的 → 直觉的~~就是不需要别人来教就懂的】 更多介绍:https://www./features/ The easiest way to plan, visualize and document your everyday molecular cloning procedures. 1. Improve accuracy while moving faster, so you save time and money. =模拟 Elegant, information-rich(信息量大) windows for simulating common cloning and PCR methods. Clear visual(视觉的,可见的;invisible无形的) schematics let you see exactly how your construct will be put together. SnapGene helps you identify and avoid common mis-steps by keeping track of details like DNA methylation and phosphorylation. 2. With unparalleled visibility and control, even novice cloners will quickly accomplish complex tasks. =可视化Gain unparalleled visibility of your DNA or protein sequence.Create and browse richly annotated plasmid maps or scan large DNA sequences with thousands of annotated features. Confirm your cloning results with highly visual alignment of sequencing results to your simulated construct.【unparalleled 】ADJ 无比的;空前的;无双的;绝无仅有的If you describe something as unparalleled, you are emphasizing that it is, for example, bigger, better, or worse than anything else of its kind, or anything that has happened before. 【novice 】 [ˈnɑːvɪs] 记忆技巧:nov 新的(novel) + ice 表人 → 新手(区分:niece侄女) 3. Automatic documentation of simulated procedures saves time and makes sharing and saving work easier, more efficient, and more effective. =自动保存 Automatically generate a rich graphical history of every sequence edit and cloning procedure Enhance collaboration and retain knowledge by sharing constructs complete with the historical details of how it was made 自动保存历史编辑 Experiment confidently with comprehensive “undo” capabilities that retrace your steps 可撤回
efficient, effective这组词都有“有效的”的意思,其区别是: efficient “效率高的”,“有能力的”,用以指人或物,着重有效地利用时间、精力并取得预期效果。如:The German telephone system is highly efficient。(德国的电话系统效率很高。) effective 常用以指物,强调能产生某种预期的效果。如:effective medicine, effective method等。 :#有的科研计划虽然在短时间内被高效地(efficiently)执行了,但是可能没什么用(effective),做完了就没有人再引用或回顾之前的研究,就连这个团队自己也没有在这个项目的基础上进行进一步研究。# |
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来自: GCTA > 《科研软件资源安装使用教程》