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Figure 1 Mouse breast carcinoma cells were purified from a mouse 4T1 tumor using the Tumor Cell Isolation Kit, mouse, an LS Column, and a QuadroMACS™ Separator. The 4T1 tumor cells were GFP-labeled prior to tumor induction for subsequent detection. After tumor dissociation and sorting, the unseparated fraction and the negative fraction were analyzed by flow cytometry for GFP and lineage markers (CD45, Ter119, and CD31 combined in the APC channel) using the MACSQuant® Analyzer. Before separation   view details Isolated mouse tumor cells  view details Figure 2 GFP-expressing mouse colon carcinoma cells (CT26.WT) were isolated from a heterogenous cell population and subsequently, the unseparated fraction and the mouse tumor cell fraction were cultured for three days in expansion medium (90% RPMI-1640, 10% FBS, 100 U/mL penicillin, and 100 U/mL streptomycin). After fixation, cells were stained using CD90.2 antibody (red) and cell nuclei were stained with DAPI (blue). The tumor cells were detected by their expression of GFP (green). Before separation  view details Isolated mouse tumor cells  view details |
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来自： 美丽人生小晚生 > 《实体肿瘤细胞分离》
