共 33 篇文章
显示摘要每页显示  条
Improving plant drought, salt, and freezing tolerance by gene transfer of a single stress-inducibleImproving plant drought, salt, and freezing tolerance by gene transfer of a single stress-inducible transcription factor.The transcription factor DREB1A specifically interacts with the DRE and induces expression of stres...
How Can Research on Plants Contribute to Promoting Human Health?Plant biochemistry can makesignificant contributions to human health through the identification andmeasurementof the many metabolites in plant-based foods,particularly those known to promote health (phytonutrients).
TITLE: Inhibition of the yeast mating pathway by Kluyveromyces lactis andPichia acaciae toxinsABSTRACTThe mating pathway of Saccharomyces cerevisiae is inhibited by the toxinsproduced by Kluyveromyces lactis and Pichia acaciae.Addition of K. lactis toxin during mating of cells that can divide in itspresence causes an ...
Win7无线网卡无法启用的解决办法这篇文章的内容如下:设备管理器显示无线网卡的驱动安装成功,也正常启用,但是在网络连接里面的无线网显示已禁用,而用无法启用。HP4416的机子,Broadcom网卡,已换了很多驱动,无线网服务也启动了,BIOS也恢复了。开始菜单-运行输入services.msc然后确定!找到WLAN Autoconfig这一项,启动此项服务,一切就OK了...
食用菌知识园地--制作技术常用的消毒药品及其配制、使用方法。2.0.1%的升汞水(HgCL3):能使蛋白质变性,抑制酶类。0.1%升汞配制方法是称取升汞0.1克,用少许酒精溶解,再加水至100毫升即成。7.漂白粉水:取漂白粉10克,加水140毫升配成。9.2%硫酸铜溶液:用硫酸铜(胆矾)2克,加水至 100毫升加热溶解配成。10.0.5%波尔多液:先用硫...
The rice mutant lazy1 also has reduced shoot gravitropism, suggesting that this gene plays a critical role in gravitropism of higher plants.Arabidopsis lazy1 exhibited reduced circumnutation of shoots though circumnutation is completely lost in rice lazy1 coleoptiles.Though rice LAZY1 is a single gene, Arabidopsis LAZ...
3''-RA CE的—般过程是:以oligo(dT)17和在许多文章中被称为锚引物(anchor primer)或接头(adaptor)的序列组成的引物QT逆转录mRNA得到第一条cDNA链,然后用含部分锚引物序列的引物Q0与基因特异性引物GSP1进行第一轮扩增得到双链cDNA ,第二轮扩增则采用内部引物(nested primer)Ql和GSP2,以防止产生非特异性扩增产物。(2)Qo和一个在GSP-...
RACE 总结RACE PCR 专题,欢迎参加讨论!然后用一个含有部分接头序列的通用引物UPM(universal primer,UPM)作为上游引物,用一个基因特异引物2(GSP 2 genespecific primer,GSP)作为下游引物,以SMART第一链cDNA为模板,进行PCR循环,把目的基因5''末端的cDNA片段扩增出来。最终,从2个有相互重叠序列的3'' / 5''-RACE产...
这个专利的方法是利用逆转录酶内源的末端转移酶活性,只要单管,一步即可完成,不需要额外的cDNA抽提纯化或者沉淀,或者额外的酶反应,只需要少至25ng的mRNA或者50ng的Total RNA就可以得到高质量、高产量的cDNA库,更重要的是得到的cDNA能够代表原有样品中的mRNA的丰度,可以应用于直接扩增基因、构建cDNA文库、已知序列钓全长cDNA(RACE),和...
帮助 | 留言交流 | 联系我们 | 服务条款 | 下载网文摘手 | 下载手机客户端
北京六智信息技术股份有限公司 Copyright© 2005-2024 360doc.com , All Rights Reserved
京ICP证090625号 京ICP备05038915号 京网文[2016]6433-853号 京公网安备11010502030377号
返回
顶部