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耐药结核病的分子生物学诊断技术

 Cheximing 2020-04-24

作者简介

01

半巢式全自动实时荧光定量PCR检测

02

线性探针检测

03

实时荧光PCR熔解曲线耐多药检测

参考文献:

1. Boehme CC, Nabeta P, Hillemann D et al. Rapid molecular detection of tuberculosis and rifampin resistance. N Engl J Med. 2010 Sep 9;363(11):1005-15.

2. Using the Xpert MTB/RIF assay to detect pulmonary and extrapulmonary tuberculosis and rifampicin resistance in adults and children. Expert Group Meeting Report, World Health Organization. 2013.

3. Policy statement: automated real-time nucleic acid amplification technology for rapid and simultaneous detection of tuberculosis and rifampicin resistance: Xpert MTB/RIF system. Geneva, World Health Organization, 2011.

4. WHO meeting report of a technical expert consultation: non-inferiority analysis of Xpert MTF/RIF Ultra compared to Xpert MTB/RIF. Geneva: World Health Organization; 2017 (WHO/HTM/TB/2017.04). Licence: CC BY-NCSA 3.0 IGO.

5.陈效友,许绍发.结核病诊断新进展.北京科学技术出版社,2017.

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