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铁氧还蛋白(FDS)通过调节电子向下游酶的分布，在光合作用中起着至关重要的作用。水稻(Oryza sativa L.)基因组中已注释了多个Fd基因，但其具体功能尚不清楚。在此，我们报道了水稻FD1的功能特性。序列比对、7个水稻FD蛋白的系统发育分析和定量逆转录聚合酶链反应(qRT-PCR)分析表明，水稻FD1是一个初级叶型FD。NADP和细胞色素c的电子转移分析表明，FD1可以将光系统I(PSI)中的电子提供给铁氧还蛋白-NADP还原酶。 功能丧失的FD1突变体在三叶期表现出黄化和幼苗致死。FD1的缺失影响了光合作用电子传递，影响了碳同化。外源葡萄糖处理部分恢复了突变体的表型，表明FD1在水稻光合作用电子传递中起重要作用。此外，fd1突变体中fd依赖基因的转录水平受到影响，其趋势与在fdc2植株中观察到的趋势相似。综上所述，这些结果表明OsFd1是水稻光合电子传递和碳同化的主要FD。 Ferredoxins (Fds) play a crucial role in photosynthesis by regulating the distribution of electrons to downstream enzymes. Multiple Fd genes have been annotated in the Oryza sativa L. (rice) genome; however, their specific functions are not well understood. Here, we report the functional characterization of rice Fd1 . Sequence alignment, phylogenetic analysis of seven rice Fd proteins and quantitative reverse transcription polymerase chain reaction (qRT‐PCR) analysis showed that rice Fd1 is a primary leaf‐type Fd. Electron transfer assays involving NADP+ and cytochrome c indicated that Fd1 can donate electrons from photosystem I (PSI) to ferredoxin‐NADP+ reductase. Loss‐of‐function fd1 mutants showed chlorosis and seedling lethality at the three‐leaf stage. The deficiency of Fd1 impaired photosynthetic electron transport, which affected carbon assimilation. Exogenous glucose treatment partially restored the mutant phenotype, suggesting that Fd1 plays an important role in photosynthetic electron transport in rice. In addition, the transcript levels of Fd‐dependent genes were affected in fd1 mutants, and the trend was similar to that observed in fdc2 plants. Together, these results suggest that OsFd1 is the primary Fd in photosynthetic electron transport and carbon assimilation in rice. 版权作品，未经PaperRSS书面授权，严禁转载，违者将被追究法律责任。 PaperRSS，关注生命科学，高校院所科研进展。
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