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实验室简介 实验室负责人骆观正教授为中山大学2017年“百人计划”引进人才，现任生命科学学院教授，国家重点实验室PI，博士生导师。长期以来从事表观遗传修饰和基因表达调控方面的研究，在生物信息学和分子生物学等方面均具有丰富的科研经验，系统研究了DNA及RNA甲基化（m6A）修饰对基因表达调控的作用机制，开发多个高通量测序和计算方法。详情可参考： http://lifesciences./teachers/professor/258 实验室致力于发现和解决关键的生物学问题，并不拘泥于某一种技术或某一个技能，提倡多学科交叉合作。每一个加入实验室的学生和科研人员都有相对独立的研究方向，彼此有关联但不重叠，每个人都有充分发展兴趣和培养技能的空间。特别欢迎对于生命的本质有着强烈探索精神的有志青年加入。实验室将提供一个相对宽松的科研文化，欢迎不同背景的同事加入，也会鼓励学习和促成合作。目前实验室经过前期建设，已经走上正轨，稳定产出结果。目前作为独立实验室已有一篇Genome Biology和一篇Cell Research发表，另有多个课题有望在近期取得突破。 岗位要求 1. 分子生物学和生物化学、生物信息学、细胞生物学等相关专业博士毕业生（或将于2019年毕业），符合中山大学博士后入站基本标准。 2. 品行端正，身心健康，工作责任心强，善于沟通，具有良好的团队精神。 3. 协助培养研究生，协助指导课题进展及撰写论文。 4. 具有NGS建库及测序、单细胞操作、酶的定向进化、化学修饰检测、基因编辑、多组学联合分析等研究经验者优先。 申请方式 有意者请将详细简历（包括学历、研究经历、发表文章等）通过电子邮件发至：luogzh5@mail.，实验室报销面试差旅费。 岗位待遇 1. 基本工资年薪20万（税前），实验室根据工作成绩提供额外奖励。 2. 学校提供校内博士后公寓，享受公费医疗，子女可安排附属幼儿园入学。 3. 协助申请广东省各类博士后人才项目，工资可有较大幅度上涨。 4. 博士后在站期限为3年，因项目进展需要可申请至4年。出站后可继续申请专职研究员岗位。优秀者可直接应聘中山大学副教授，或广东省其它高校同等职位。 研究方向 1. NGS测序及数据分析相关技术开发：围绕DNA和RNA上新型表观修饰如m6A等热点问题，开发新的测序方法和分析技术，发现新的基因表达调控规律。 2. 干细胞重编程机制：利用单细胞测序等前沿技术，研究干细胞去分化、转分化等重编程过程中的表观遗传变化规律，阐明细胞命运决定机制；探索医学应用潜力。 3. 表观遗传组和基因转录调控：从表观遗传角度，探索基因表达调控基础和普遍的机理。 代表性论文： 2018 N6-methyldeoxyadenosine directs nucleosome positioning inTetrahymena DNA. Genome Biology. 19:200 (2018) 2017 Luo GZ & He C. DNA N6-methyladenine in metazoans: functional epigenetic mark or bystander? Nature Structural & Molecular Biology. 24(6):503-506 (2017) Roundtree IA, Luo GZ, Zhang Z, Wang X, Zhou T, Cui Y, Sha J, Huang X, Guerrero L, Xie P, He E, Shen B, He C. YTHDC1 mediates nuclear export of N6-methyladenosine methylated mRNAs. Elife. pii: e31311 (2017) Hsu PJ, Zhu Y, Ma H, Guo Y, Shi X, Liu Y, Qi M, Lu Z, Shi H, Wang J, Cheng Y, Luo G, Dai Q, Liu M, Guo X, Sha J, Shen B, He C. Ythdc2 is an N6-methyladenosine binding protein that regulates mammalian spermatogenesis. Cell Research. 27(9):1115-1127 (2017) 2016 Liu J#, Zhu Y#, Luo GZ#, Wang X#, Yue Y, Wang X, Zong X, Chen K, Yin H, Fu Y, Han D, Wang Y, Chen D, He C. Abundant DNA 6mA methylation during early embryogenesis of zebrafish and pig. Nature Communications.7:13052 (2016) Luo GZ, Wang F, Weng X, Chen K, Hao Z, Yu M, Deng X, Liu J, He C. Characterization of eukaryotic DNA N6-methyladenine by a highly sensitive restriction enzyme-assisted sequencing. Nature Communications. 7:11301 (2016) Liu F, Clark W, Luo G, Wang X, Fu Y, Wei J, Wang X, Hao Z, Dai Q, Zheng G, Ma H, Han D, Evans M, Klungland A, Pan T, He C. ALKBH1-Mediated tRNA Demethylation Regulates Translation. Cell. 167(3): 816-828 (2016) Feng G, Tong M, Xia B, Luo GZ, Wang M, Xie D, Wan H, Zhang Y, Zhou Q, Wang XJ. Ubiquitously expressed genes participate in cell-specific functions via alternative promoter usage. EMBO Reports. 17(9):1304-13 (2016) 2015 Luo GZ, Blanco MA, Greer EL, He C, Shi Y. DNA N6-methyladenine: a new epigenetic mark in eukaryotes? Nature Reviews Molecular Cell Biology. 16(12):705-10 (2015) Fu Y#, Luo GZ#, Chen K, Deng X, Yu M, Han D, Hao Z, Liu J, Lu X, Doré LC, Weng X, Ji Q, Mets L, He C. N6-Methyldeoxyadenosine Marks Active Transcription Start Sites in Chlamydomonas. Cell. 161(4):879-92 (2015) (# co-first author). Chen K, Luo GZ, He C. High-Resolution Mapping of N⁶-Methyladenosine in Transcriptome and Genome Using a Photo-Crosslinking-Assisted Strategy. Methods Enzymol. 560:161-85 (2015) Shuai L, Wang Y, Dong M, Wang X, Sang L, Wang M, Wan H, Luo G, Gu T, Yuan Y, Feng C, Teng F, Li W, Liu X, Li T, Wang L, Wang XJ, Zhao XY, Zhou Q. Durable pluripotency and haploidy in epiblast stem cells derived from haploid embryonic stem cells in vitro. J Mol Cell Biol. 7(4):326-37 (2015) Deng X, Chen K, Luo GZ, Weng X, Ji Q, Zhou T, He C. Widespread occurrence of N6-methyladenosine in bacterial mRNA.Nucleic Acids Research. 43(13):6557-67 (2015) Chen K, Lu Z, Wang X, Fu Y, Luo GZ, Liu N, Han D, Dominissini D, Dai Q, Pan T and He C. High-Resolution N6-Methyladenosine (m6A) Map Using Photo-Crosslinking-Assisted m6A Sequencing. Angew Chem Int Ed Engl. 54(5):1587-90 (2015) 2014 Luo GZ, MacQueen A, Zheng G, Duan H, Dore LC, Lu Z, Liu J, Chen K, Jia G, Bergelson J, He C. Unique features of the m6A methylome in Arabidopsis thaliana. Nature Communications. 5:5630 (2014) Luo GZ, Yang W, Ma YK, Wang XJ. ISRNA: an integrative online toolkit for short reads from high-throughput sequencing data. Bioinformatics. 30(3):434-6 (2014) Li W, Li X, Li T, Jiang MG, Wan H, Luo GZ, Feng C, Cui X, Teng F, Yuan Y, Zhou Q, Gu Q, Shuai L, Sha J, Xiao Y, Wang L, Liu Z, Wang XJ, Zhao XY, Zhou Q. Genetic modification and screening in rat using haploid embryonic stem cells. Cell Stem Cell. 14(3):404-14 (2014) Fu L, Shi Z, Luo G, Tu W, Wang X, Fang Z, Li X. Multiple microRNAs regulate human FOXP2 gene expression by targeting sequences in its 3' untranslated region. Mol Brain. 7:71 (2014) Before 2013 Luo GZ, Hafner M, Shi Z, Brown M, Feng GH, Tuschl T, Wang XJ, Li X. Genome-wide annotation and analysis of zebra finch microRNA repertoire reveal sex-biased expression. BMC Genomics. 13:727 (2012) Liu L#, Luo GZ#, Yang W#, Zhao X#, Zheng Q, Lv Z, Li W, Wu HJ, Wang L, Wang XJ, Zhou Q. Activation of the imprinted Dlk1-Dio3 region correlates with pluripotency levels of mouse stem cells. Journal of Biological Chemistry. 285(25):19483-90 (2010) (# co-first author). Shi Z, Luo G, Fu L, Fang Z, Wang X, Li X. miR-9 and miR-140-5p target FoxP2 and are regulated as a function of the social context of singing behavior in zebra finches. J Neurosci. 33(42):16510-21 (2013) Wan H, He Z, Dong M, Gu T, Luo GZ, Teng F, Xia B, Li W, Feng C, Li X, Li T, Shuai L, Fu R, Wang L, Wang XJ, Zhao XY, Zhou Q. Parthenogenetic haploid embryonic stem cells produce fertile mice. Cell Research. 23(11):1330-3 (2013) Zhang Y, Teng F, Luo GZ, Wang M, Tong M, Zhao X, Wang L, Wang XJ, Zhou Q. MicroRNA-323-3p regulates the activity of polycomb repressive complex 2 (PRC2) via targeting the mRNA of embryonic ectoderm development (Eed) gene in mouse embryonic stem cells. J Biol Chem. 288(33):23659-65 (2013) Li RC, Tao J, Guo YB, Wu HD, Liu RF, Bai Y, Lv ZZ, Luo GZ, Li LL, Wang M, Yang HQ, Gao W, Han QD, Zhang YY, Wang XJ, Xu M, Wang SQ. In Vivo Suppression of MiR-24 Prevents the Transition toward Decompensated Hypertrophy in Aortic-constricted Mice. Circ Res. 112(4):601-5 (2013) Han J, Chen L, Luo G, Dai B, Wang X, Dai J. Three-dimensional culture may promote cell reprogramming. Organogenesis. 9(2):118-20 (2013) Liu X, Zhang Y, Tong M, Liu XY, Luo GZ, Xie DF, Ren SF, Bai DH, Wang L, Zhou Q, Wang XJ. Identification of a small molecule 1,4-bis-[4-(3-phenoxy-propoxy)-but-2-ynyl]-piperazine as a novel inhibitor of the transcription factor p53. Acta Pharmacol Sin. 34(6):805-10 (2013) Li W, Shuai L, Wan H, Dong M, Wang M, Sang L, Feng C, Luo GZ, Li T, Li X, Wang L, Zheng QY, Sheng C, Wu HJ, Liu Z, Liu L, Wang L, Wang XJ, Zhao XY, Zhou Q. Androgenetic haploid embryonic stem cells produce live transgenic mice. Nature.490(7420):407-11 (2012) Xu M, Wu HD, Li RC, Zhang HB, Wang M, Tao J, Feng XH, Guo YB, Li SF, Lai ST, Zhou P, Li LL, Yang HQ, Luo GZ, Bai Y, Xi JJ, Gao W, Han QD, Zhang YY, Wang XJ, Meng X, Wang SQ. Mir-24 Regulates Junctophilin-2 Expression in Cardiomyocytes. Circ Res. 111(7):837-41 (2012) Zhang C, Xu Y, Guo S, Zhu J, Huan Q, Liu H, Wang L, Luo G, Wang X, Chong K. Dynamics of brassinosteroid response modulated by negative regulator LIC in rice. PLoS Genet. 8(4):e1002686 (2012) Du Y, Gao C, Liu Z, Wang L, Liu B, He F, Zhang T, Wang Y, Wang X, Xu M, Luo GZ, Zhu Y, Xu Q, Wang X, Kong W. Upregulation of a disintegrin and metalloproteinase with thrombospondin motifs-7 by miR-29 repression mediates vascular smooth muscle calcification. Arterioscler Thromb Vasc Biol. 32(11):2580-8 (2012) 详细论文列表及下载可参考Google scholar: https://scholar.google.com/citations?user=wqUHZnEAAAAJ&hl=en |
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