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E-cigarettes compromise the gut barrier and trigger inflammation 10.1016/j.isci.2021.102035 01-05, Article Abstract & Authors:展开 Abstract:收起
E-cigarette usage continues to rise, yet the safety of e-cigarette aerosols is questioned. Using murine models of acute and chronic e-cigarette aerosol inhalation, murine colon transcriptomics and murine and human gut-derived organoids in co-culture models, we assessed the effects of e-cigarette use on the gut barrier. Histologic and transcriptome analyses revealed that chronic, but not acute, nicotine-free e-cigarette use increased inflammation and reduced expression of tight junction markers (TJ). Exposure of murine and human enteroid-derived monolayers (EDMs) to nicotine-free e-cigarette aerosols alone or in co-culture with bacteria, also cause barrier-disruption, downregulation of TJ protein, enhanced inflammation in response to infection. These data highlight the harmful effect of “non-nicotine” component of e-cigarette on gut barrier. Considering the importance of an intact gut barrier for host fitness, and the impact of gut mucosal inflammation on a multitude of chronic diseases, these findings are broadly relevant to both medicine and public health.
First Authors:
Aditi Sharma,Jasper Lee,Ayden G Fonseca Correspondence Authors:
Laura E Crotty-Alexander,Pradipta Ghosh,Soumita Das All Authors:
Aditi Sharma,Jasper Lee,Ayden G Fonseca,Alex Moshensky,Taha Kothari,Ibrahim M Sayed,Stella-Rita Ibeawuchi,Rama F Pranadinata,Jason Ear,Debashis Sahoo,Laura E Crotty-Alexander,Pradipta Ghosh,Soumita Das
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