看到于2020年11月发表在杂志《nature cancer》的文章:《Mutations in BRCA1 and BRCA2 differentially affect the tumor microenvironment and response to checkpoint blockade immunotherapy》里面有全基因组测序数据,文献链接是:https://www./articles/s43018-020-00139-8
DNA was extracted and sequenced at 74–106× (median 80×) coverage.
FASTQ files were aligned to MM10 mouse reference with bwamem v.0.7.15.
Somatic mutations were called with VarScan v.2.4.3, Strelka v.2.9.10, Platypus 0.8.1, Mutect2 (part of GATK 4.1.4.1) and SomaticSniper 1.0.5.0 (for SNVs only).
DNA from a 4T1 parental single-cell clone at time 0 was used as normal.