we performed deep single cell RNA sequencing to 7,066 immune cells collected from 8 TNBC patients using the MIRALCS platform. We identified 13 immune cell subsets according to their transcriptome features. We found that the frequency of identified T cells and macrophages varied greatly across patients.
Here we report single-cell RNA sequencing results of 9683 tumor-infiltrated immune cells isolated from 14 treatment naïve TNBC tumors, where 22 immune cell subsets
此方法学的文章: Wu et al. Full-length single-cell RNA-seq applied to a viral human cancer: applications to HPV expression and splicing analysis in HeLa S3 cells. GigaScience (2015) 4:51.