Impact of the Human Cell Atlas on medicine.
Rood JE, Maartens A, Hupalowska A, Teichmann SA, Regev A. Nat Med. 2022 Dec 8. doi: 10.1038/s41591-022-02104-7.
Online ahead of print. PMID: 36482102 Review.
Identifying disease-critical cell types and cellular processes by integrating single-cell RNA-sequencing and human genetics.
Jagadeesh KA, Dey KK, Montoro DT, Mohan R, Gazal S, Engreitz JM, Xavier RJ, Price AL, Regev A. Nat Genet. 2022 Oct;54(10):1479-1492. doi: 10.1038/s41588-022-01187-9. Epub 2022 Sep 29. PMID: 36175791
Mostly natural sequencing-by-synthesis for scRNA-seq using Ultima sequencing.
Simmons SK, Lithwick-Yanai G, Adiconis X, Oberstrass F, Iremadze N, Geiger-Schuller K, Thakore PI, Frangieh CJ, Barad O, Almogy G, Rozenblatt-Rosen O, Regev A, Lipson D, Levin JZ. Nat Biotechnol. 2022 Sep 15. doi: 10.1038/s41587-022-01452-6. Online ahead of print. PMID: 36109685
Single-nucleus cross-tissue molecular reference maps toward understanding disease gene function.
Eraslan G, Drokhlyansky E, Anand S, Fiskin E, Subramanian A, Slyper M, Wang J, Van Wittenberghe N, Rouhana JM, Waldman J, Ashenberg O, Lek M, Dionne D, Win TS, Cuoco MS, Kuksenko O, Tsankov AM, Branton PA, Marshall JL, Greka A, Getz G, Segrè AV, Aguet F, Rozenblatt-Rosen O, Ardlie KG, Regev A. Science. 2022 May 13;376(6594):eabl4290. doi: 10.1126/science.abl4290. Epub 2022 May 13. PMID: 35549429
Spatial components of molecular tissue biology.
Palla G, Fischer DS, Regev A, Theis FJ. Nat Biotechnol. 2022 Mar;40(3):308-318. doi: 10.1038/s41587-021-01182-1. Epub 2022 Feb 7. PMID: 35132261
Massively parallel phenotyping of coding variants in cancer with Perturb-seq.
Ursu O, Neal JT, Shea E, Thakore PI, Jerby-Arnon L, Nguyen L, Dionne D, Diaz C, Bauman J, Mosaad MM, Fagre C, Lo A, McSharry M, Giacomelli AO, Ly SH, Rozenblatt-Rosen O, Hahn WC, Aguirre AJ, Berger AH, Regev A, Boehm JS. Nat Biotechnol. 2022 Jun;40(6):896-905. doi: 10.1038/s41587-021-01160-7. Epub 2022 Jan 20. PMID: 35058622
KOL之单细胞开拓者：fuchou tang
单细胞三代测序、非编码测序，让2022年的单细胞技术更丰富。
Molecular profiling of human non-small cell lung cancer by single-cell RNA-seq.
Li Q, Wang R, Yang Z, Li W, Yang J, Wang Z, Bai H, Cui Y, Tian Y, Wu Z, Guo Y, Xu J, Wen L, He J, Tang F, Wang J. Genome Med. 2022 Aug 13;14(1):87. doi: 10.1186/s13073-022-01089-9. PMID: 35962452
KOL之肿瘤异质性探秘者：zemin zhang
为我们贡献了肿瘤中性粒细胞，这一2022细胞明星。
Liver tumour immune microenvironment subtypes and neutrophil heterogeneity.
Xue R, Zhang Q, Cao Q, Kong R, Xiang X, Liu H, Feng M, Wang F, Cheng J, Li Z, Zhan Q, Deng M, Zhu J, Zhang Z, Zhang N. Nature. 2022 Dec;612(7938):141-147. doi: 10.1038/s41586-022-05400-x. Epub 2022 Nov 9. PMID: 36352227
Immune phenotypic linkage between colorectal cancer and liver metastasis.
Liu Y, Zhang Q, Xing B, Luo N, Gao R, Yu K, Hu X, Bu Z, Peng J, Ren X, Zhang Z. Cancer Cell. 2022 Apr 11;40(4):424-437.e5. doi: 10.1016/j.ccell.2022.02.013. Epub 2022 Mar 17. PMID: 35303421
KOL之单细胞工具集成者： Satija R
2022年，您的Seurat更新了吗？
Comparison and evaluation of statistical error models for scRNA-seq. Choudhary S, Satija R. Genome Biol. 2022 Jan 18;23(1):27. doi: 10.1186/s13059-021-02584-9. PMID: 35042561
SCENIC+: single-cell multiomic inference of enhancers and gene regulatory networks
Aerts, S. How regulatory sequences learn cell representations. Nat Methods 19, 1041–1043 (2022). https:///10.1038/s41592-022-01570-8
Cell type diversity in a developing octopus brain.
Styfhals R, Zolotarov G, Hulselmans G, Spanier KI, Poovathingal S, Elagoz AM, De Winter S, Deryckere A, Rajewsky N, Ponte G, Fiorito G, Aerts S, Seuntjens E. Nat Commun. 2022 Nov 30;13(1):7392. doi: 10.1038/s41467-022-35198-1. PMID: 36450803
Decoding gene regulation in the fly brain.
Janssens J, Aibar S, Taskiran II, Ismail JN, Gomez AE, Aughey G, Spanier KI, De Rop FV, González-Blas CB, Dionne M, Grimes K, Quan XJ, Papasokrati D, Hulselmans G, Makhzami S, De Waegeneer M, Christiaens V, Southall T, Aerts S. Nature. 2022 Jan;601(7894):630-636. doi: 10.1038/s41586-021-04262-z. Epub 2022 Jan 5. PMID: 34987221
KOL之单细胞自然绘手：guoji guo
在单细胞多物种和跨物种研究中，带领人们领略大自然之美。
Construction of a cross-species cell landscape at single-cell level. Wang R, Zhang P, Wang J, Ma L, E W, Suo S, Jiang M, Li J, Chen H, Sun H, Fei L, Zhou Z, Zhou Y, Chen Y, Zhang W, Wang X, Mei Y, Sun Z, Yu C, Shao J, Fu Y, Xiao Y, Ye F, Fang X, Wu H, Guo Q, Fang X, Li X, Gao X, Wang D, Xu PF, Zeng R, Xu G, Zhu L, Wang L, Qu J, Zhang D, Ouyang H, Huang H, Chen M, Ng SC, Liu GH, Yuan GC, Guo G, Han X. Nucleic Acids Res. 2022 Aug 5:gkac633. doi: 10.1093/nar/gkac633. Online ahead of print. PMID: 35929025
SPEED: Single-cell Pan-species atlas in the light of Ecology and Evolution for Development and Diseases.
Chen Y, Zhang X, Peng X, Jin Y, Ding P, Xiao J, Li C, Wang F, Chang A, Yue Q, Pu M, Chen P, Shen J, Li M, Jia T, Wang H, Huang L, Guo G, Zhang W, Liu H, Wang X, Chen D. Nucleic Acids Res. 2022 Oct 28:gkac930. doi: 10.1093/nar/gkac930. Online ahead of print. PMID: 36305818
KOL之推开空间组学之门： Fan Rong
在空间尺度上，逼近单细胞真相。
Spatial profiling of chromatin accessibility in mouse and human tissues .
Deng Y, Bartosovic M, Ma S, Zhang D, Kukanja P, Xiao Y, Su G, Liu Y, Qin X, Rosoklija GB, Dwork AJ, Mann JJ, Xu ML, Halene S, Craft JE, Leong KW, Boldrini M, Castelo-Branco G, Fan R. Nature. 2022 Sep;609(7926):375-383. doi: 10.1038/s41586-022-05094-1. Epub 2022 Aug 17. PMID: 35978191
Spatial-CITE-seq: spatially resolved high-plex protein and whole transcriptome co-mapping.
Fan R, Liu Y, DiStasio M, Su G, Asashima H, Enninful A, Qin X, Deng Y, Bordignon P, Cassano M, Tomayko M, Xu M, Halene S, Craft J, Hafler D. Res Sq. 2022 Mar 30:rs.3.rs-1499315. doi: 10.21203/rs.3.rs-1499315/v1. Preprint. PMID: 35378748
Single-cell antigen-specific landscape of CAR T infusion product identifies determinants of CD19-positive relapse in patients with ALL.
Bai Z, Woodhouse S, Zhao Z, Arya R, Govek K, Kim D, Lundh S, Baysoy A, Sun H, Deng Y, Xiao Y, Barrett DM, Myers RM, Grupp SA, June CH, Fan R, Camara PG, Melenhorst JJ. Sci Adv. 2022 Jun 10;8(23):eabj2820. doi: 10.1126/sciadv.abj2820. Epub 2022 Jun 8. PMID: 35675405
KOL之单细胞微生物见者：David Weitz
以单细胞技术操作单细胞生物，将单细胞测序技术应用到丰富多样的微生物世界。
High-throughput, single-microbe genomics with strain resolution, applied to a human gut microbiome .
Zheng W, Zhao S, Yin Y, Zhang H, Needham DM, Evans ED, Dai CL, Lu PJ, Alm EJ, Weitz DA. Science. 2022 Jun 3;376(6597):eabm1483. doi: 10.1126/science.abm1483. Epub 2022 Jun 3. PMID: 35653470
Large-scale single-cell encapsulation in microgels through metastable droplet-templating combined with microfluidic-integration.
Zhang H, Zhang L, An C, Zhang Y, Shao F, Gao Y, Zhang Y, Li H, Zhang Y, Ren C, Sun K, He W, Cheng F, Wang H, Weitz DA. Biofabrication. 2022 Jun 6;14(3). doi: 10.1088/1758-5090/ac7168. PMID: 35593920
KOL之单细胞分析加速神器： Fabian Theis
scanpy 开发者之一，为海量单细胞数据分析提供解决方案。
Palla, G., Spitzer, H., Klein, M. et al. Squidpy: a scalable framework for spatial omics analysis . Nat Methods 19, 171–178 (2022). https:///10.1038/s41592-021-01358-2
