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In vivo SilenceMag™ is a rapid, simple and highly efficient method dedicated to transfect small RNA (siRNA, miRNA) into target cells/tissue in vivo. It combines magnetic nanoparticles and small RNA that will be retained after injection at the magnetically targeted site. This targeted delivery method minimizes systemic distribution, increases gene targeted inactivation and reduces toxicity. Furthermore, the magnetic forces enhance the uptake of magnetic nanoparticles by the target tissue, and thus improve the efficiency of silencing. This allows decreasing the required process time of delivery to few minutes which is crucial for improvement of in vivo small RNA delivery. In vivo SilenceMag™ is designed to meet in vivo grade quality. In vivo SilenceMag™ benefits: Increased silencing efficiency Targeted process (magnetically-driven) Reduction of the systemic dissemination of siRNA/miRNA during injection Reduction of the siRNA/miRNA doses Work under non permissive conditions (hypothermia, physiological flow conditions) Penetration of the siRNA/miRNA into tissues Minimized toxicity IN VIVO SILENCEMAG TRIAL试剂盒#IV-TK30240IN VIVO SILENCEMAG TRIAL试剂盒是一种快速、简单且高效的方法，致力于将小 RNA（siRNA、miRNA）转染到体内靶细胞/组织中。它结合了磁性纳米颗粒和小RNA，在磁性靶向位点注射后将保留。这种靶向递送方法极大限度地减少了全身分布，增加了基因靶向失活并降低了毒性。此外，磁力增强了靶组织对磁性纳米颗粒的吸收，从而提高了SILENCEMAG效率。 这样可以将所需的递送过程时间减少到几分钟，这对于改善体内小RNA递送至关重要。体内SilenceMag™旨在满足体内级质量。 IN VIVO SILENCEMAG TRIAL试剂盒特点： 提高消音效率 目标工艺（磁力驱动） 减少注射过程中siRNA/miRNA的全身性传播 减少siRNA/miRNA剂量 在非许可条件下工作（体温过低、生理流动条件） siRNA/miRNA渗透到组织中 毒性最小化 IN VIVO SILENCEMAG TRIAL试剂盒结果示例： 小鼠皮下肿瘤转染。 Chen和她的合作者已经表明，使用体内SilenceMag与外部磁场的应用相结合，增加了针对肿瘤部位血管内皮生长因子（VEGF）的siRNA的吸引力和保留。通过SPECT和MRI评估siRNA / SilenceMag的生物分布和细胞毒性，并每天评估肿瘤大小（Chen T，Zhu S，Tong L，Li J，Chen F，Han Y，Zhao Y，Xiong W. 2014 超顺磁性氧化铁纳米颗粒介导的131I-hVEGF siRNA抑制裸鼠肝细胞癌肿瘤生长。BMC 癌症。14:414). 
图：通过将肝癌肿瘤细胞注射到免疫抑制小鼠的右胁中产生皮下肿瘤。然后每天在静脉注射VEGF / SilenceMag后监测肿瘤生长。来自Chen等人，BMC Cancer，2014;14:114.
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