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马卉1 张润田1 李航2 黄敏1 陈广山1 刘洋3 曲天歌4 包海兰5 周琳1 赵艳1 段行武1 李玲玲1 1 北京中医药大学东直门医院皮肤科，北京，100700；2 北京大学第一医院皮肤性病科，北京，100034；3 首都医科大学附属北京世纪坛医院， 北京，100038；4 北京中医药大学东方医院教育处，北京，100078；5 内蒙古自治区人民医院中西医结合科，呼和浩特，010017  摘要 目的：研究不同浓度龙血素A对瘢痕疙瘩成纤维细胞增殖、凋亡、迁移能力的影响以及对Col-Ⅰ、Col-Ⅲ、α-SMA、Bax、Caspase-3蛋白表达水平的影响。方法：1）不同浓度龙血素A作用于瘢痕疙瘩成纤维细胞，用CellTiter-Blue活力检测试验检测龙血素A对瘢痕疙瘩成纤维细胞增殖的影响。2）流式细胞术检测龙血素A对瘢痕疙瘩成纤维细胞凋亡的影 响。3）划痕实验检测龙血素A对瘢痕疙瘩成纤维细胞迁移的影响。4）Western-Blot测定龙血素A对瘢痕疙瘩成纤维细胞Col-Ⅰ、Col-Ⅲ、α-SMA、Bax、Caspase-3蛋白水平的影响。结果：龙血素A可抑制瘢痕疙瘩成纤维细胞增殖、迁移并诱导其凋亡，同时减少COL-Ⅰ、COL-Ⅲ、α-SMA蛋白表达，并促进Bax、Caspase-3蛋白表达，并且均呈现剂量依赖性。结论：龙血素A可以调控瘢痕疙瘩成纤维细胞增殖与凋亡的平衡、减少细胞外基质沉积，从而可能对KD发挥一定的治疗作用。 关键词 龙血素A；瘢痕疙瘩；成纤维细胞 Study on the Effects of Loureirin A on Keloid Fibroblast Growth Function MA Hui1, ZHANG Runtian1, LI Hang2, HUANG Min1, CHEN Guangshan1, LIU Yang3, QU Tiange4, BAO Hailan5, ZHOU Lin1, ZHAO Yan1, DUAN Xingwu1, LI Lingling1 1 Department of Dermatology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, 100700，China；2 Department of Dermatology, Peking University First Hospital, Beijing, 100034，China；3 Beijing Shijitan Hospital Affiliated to Capital Medical University, Beijing 100038，China；4 Dongfang Hospital of Beijing University of Traditional Chinese Medicine, Beijing 100078，China；5 Department of Integrated Traditional Chinese and Western Medicine, People's Hospital of Inner Mongolia Autonomous Region, Hohhot 010017，China Abstract Objective:To study the effects of different concentrations of Loureirin A on the proliferation, apoptosis and migration of keloid fibroblasts and the protein expression levels of Col-Ⅰ, Col-Col-Ⅰ,α-SMA, Bax, Caspase-3. Methods：1）Different concentrations of L-Auxin A act on keloid fibroblasts. CellTiter-Blue vitality test was used to test the effects of L-Auxin a on keloid fibroblast proliferation. 2）Flow cytometry was used to detect the effects of L-Auxin A on keloid fibroblasts apoptosis. 3）Scratch test was used to detect the effects of borneol A on keloid fibroblast migration. 4）Proteins expression levels of Col-Ⅰ, Col-Ⅲ, α-SMA, Bax Caspase-3 were detected by Western-Blot. Results:Loureirin A can inhibit the proliferation and migration of keloid fibroblasts, an induce their apoptosis, reduce the protein expression of Col-Ⅰ, Col-Ⅲ α-SMA and promote the protein expression of Bax and caspase-3， and there is a dosage dependent relationship. Conclusion:Loureirin A can regulate the balance of keloid fibroblast proliferation and apoptosis, reduce the deposition of extracellular matrix, so it may play a certain therapeutic effects on keloid. Keywords Loureirin A；Keloid；Fibroblast |
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