李健斌,江泽飞
军事医学科学院附属医院乳腺肿瘤科 尽管循环肿瘤细胞(CTC)的发现已有较长时间,但自第一台用于检测CTC的CellSearch平台上市之后,CTC才开始得到重视并深入研究。目前,CTC已经从单纯的计数走向分子分型时代,随着测序技术的发展和液体活检需求的提高,利用CTC行单细胞测序已成为可能。如何充分利用CTC揭示肿瘤的发生与发展,判断患者的生存和预后已经成为关注的热点。 Zhonghua Zhong Liu Za Zhi. 2016;38(12):881-885. Research progress of circulating tumor cells: the way from counting to subtyping. Li JB, Jiang ZF. Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China. Although circulating tumor cells(CTCs) have long been identified, it was not until the approval of CellSearch system that CTCs are current lyunder in-depth investigation.The CTCs technology has advanced from simple cell counting into era of molecular subtyping. The development of sequencing technologies and liquid biopsy has made it possible to sequence single cell genomes. In the recent years, how to take advantage of CTCs as a useful tool to clarify the mechanisms of carcinogenesis and cancer development and to predict the patient survival have become a hot issue. PMID: 27998462 DOI: 10.3760/cma.j.issn.0253-3766.2016.12.001 参考文献 Chen W, Zheng R, Baade PD, et al. Cancer statistics in China, 2015. CA Cancer J Clin. 2016;66(2):115-132. DOI: 10.3322/caac.21338 Zhang C, Guan Y, Sun Y, et al. Tumor heterogeneity and circulating tumor cells. Cancer Lett. 2016;374(2):216-223. DOI: 10.1016/j.canlet.2016.02.024 Gorges TM, Penkalla N, Schalk T, et al. Enumeration and molecular characterization of tumor cells in lung cancer patients using a novel in vivo device for capturing circulating tumor cells. Clin Cancer Res. 2016;22(9):2197-2206. DOI: 10.1158/1078-0432.CCR-15-1416 Marcucci F, Stassi G, DE maria R. Epithelial-mesenchymal transition: a new target in anticancer drug discovery. Nat Rev Drug Discov. 2016;15(5):311-325. DOI: 10.1038/nrd.2015.13 Satelli A, Brownlee Z, Mitra A, et al. Circulating tumor cell enumeration with a combination of epithelial cell adhesion molecule- and cell-surface vimentin-based methods for monitoring breast cancer therapeutic response. Clin Chem. 2015;61(1):259-266. DOI: 10.1373/clinchem.2014.228122 穆洪, 林凯璇, 赵晓航, 等. 肝细胞肝癌外周血循环肿瘤细胞的检测. 中华肿瘤杂志. 2014;36(4):276-281. DOI: 10.3760/cma.j.issn.0253-3766.2014.04.008 Yu N, Zhou J, Cui F, et al. Circulating tumor cells in lung cancer: detection methods and clinical applications. Lung. 2015;193(2):157-171. DOI: 10.1007/s00408-015-9697-7 Hvichia GE, Parveen Z, Wagner C, et al. A novel microfluidic platform for size and deformability based separation and the subsequent molecular characterization of viable circulating tumor cells. Int J Cancer. 2016;138(12):2894-2904. DOI: 10.1002/ijc.30007 Allard WJ, Terstappen LW. CCR 20th anniversary commentary:paving the way for circulating tumor cells. Clin Cancer Res. 2015;21(13):2883-2885. DOI: 10.1158/1078-0432.CCR-14-2559 Jiang ZF, Cristofanilli M, Shao ZM, et al. Circulating tumor cells predict progression-free and overall survival in Chinese patients with metastatic breast cancer, HER2-positive or triple-negative (CBCSG004): a multicenter, double-blind, prospective trial. Ann Oncol. 2013;24(11):2766-2772. DOI: 10.1093/annonc/mdt246 边莉, 刘毅, 王涛, 等. 循环肿瘤细胞动态变化对转移性乳腺癌患者治疗反应及预后的预测价值. 中华医学杂志. 2014;94(4):265-268. DOI: 10.3760/cma.j.issn.0376-2491.2014.04.007 时志龙, 张妤, 殷正丰. 靶向循环肿瘤细胞:防治肝癌术后复发转移的新策略. 中华肿瘤杂志. 2014;36(6):401-404. DOI: 10.3760/cma.j.issn.0253-3766.2014.06.001 Liu Y, Liu Q, Wang T, et al. Circulating tumor cells in HER2-positive metastatic breast cancer patients: a valuable prognostic and predictive biomarker. BMC Cancer. 2013;13:202. DOI: 10.1186/1471-2407-13-202 Zhang S, Li L, Wang T, et al. Real-time HER2 status detected on circulating tumor cells predicts different outcomes of anti-HER2 therapy in histologically HER2-positive metastatic breast cancer patients. BMC Cancer. 2016;16(1):526. DOI: 10.1186/s12885-016-2578-5 Wang T, Zhou J, Zhang S, et al. Meaningful interpretation of serum HER2 ECD levels requires clear patient clinical background, and serves several functions in the efficient management of breast cancer patients. Clin Chim Acta. 2016;458:23-29. DOI: 10.1016/j.cca.2016.04.025 Alix-Panabières C, Pantel K. Clinical applications of circulating tumor cells and circulating tumor DNA as liquid biopsy. Cancer Discov. 2016;6(5):479-491. DOI: 10.1158/2159-8290.CD-15-1483 Bidard FC, Proudhon C, Pierga JY. Circulating tumor cells in breast cancer. Mol Oncol. 2016;10(3):418-430. DOI: 10.1016/j.molonc.2016.01.001 Grün D, van Oudenaarden A. Design and analysis of single-cell sequencing experiments. Cell. 2015;163(4):799-810. DOI: 10.1016/j.cell.2015.10.039
原文参见:中华肿瘤杂志. 2016;38(12):881-885.
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