|
表1 荧光基团的激发、发射波长及推荐的淬灭基团
| 发射光颜色 |
Dye |
Abs (nm) |
Em (nm) |
Taqman
对应淬灭基团 |
MGB探针
淬灭基因 |
分子信标
淬灭基因 |
|
AMCA |
350 |
440 |
|
|
|
|
Atto425 |
436 |
484 |
BHQ-1 |
|
Dabcyl |
|
Alexa Fluor 488 |
490 |
525 |
BHQ-1 |
MGB |
Dabcyl |
|
6-FAM |
494 |
518 |
BHQ-1 |
MGB |
Dabcyl |
|
TET |
521 |
536 |
BHQ-1 |
MGB |
Dabcyl |
|
JOE |
520 |
548 |
BHQ-1 |
MGB |
Dabcyl |
|
VIC |
538 |
554 |
BHQ-1 |
MGB |
Dabcyl |
|
HEX |
535 |
556 |
BHQ-1 |
|
Dabcyl |
|
NED |
546 |
575 |
BHQ-2 |
|
|
|
Cy3 |
552 |
570 |
BHQ-2 |
|
|
|
TAMRA |
565 |
580 |
BHQ-2 |
|
|
|
ROX |
585 |
605 |
BHQ-2 |
|
|
|
Texas red |
595 |
615 |
BHQ-2 |
|
|
|
Cy5 |
643 |
667 |
BHQ-3 |
|
|
|
Quasar 670 |
647 |
667 |
BHQ-3 |
|
|
|
Cy5.5 |
684 |
710 |
BHQ-3 |
|
|
|
Cy7 |
749 |
767 |
|
|
|
备注：上方波长数据为理论值，在不同的Oligo中，波长可能发生不同程度的偏移
表2 常用荧光定量PCR仪做多重qPCR检测时支持的荧光基团
| 仪器型号 |
染料1 |
染料2 |
染料3 |
染料4 |
染料5 |
| Agilent MX3000P/3005P/4000 |
FAM |
HEX/VIC/JOE/TET |
Cy3 |
Texas red/TEX-615 |
Cy5 |
| ABI 7300 |
FAM |
HEX/VIC/JOE |
|
|
|
| ABI 7500 FAST |
FAM |
HEX/VIC/JOE |
Cy3 |
Texas red/TEX-615 |
Cy5 |
| ABI 7900 HT |
FAM |
HEX/VIC/JOE/TET |
|
|
|
| ABI QuantStudio?系列 |
FAM |
HEX/VIC/JOE/TET |
Cy3 |
Texas red/TEX-615 |
Cy5 |
| ABI StepOne |
FAM |
HEX/VIC/JOE/TET |
|
|
|
| ABI StepOnePlus |
FAM |
HEX/VIC/JOE |
TAMRA |
|
|
| ABI ViiA?7 |
FAM |
HEX/VIC/JOE/TET |
|
Texas red/TEX-615 |
Cy5 |
| Bio-Rad CFX 96/384 |
FAM |
HEX/VIC/JOE/TET |
|
Texas red/TEX-615 |
Cy5 |
| Bio-Rad iCycler |
FAM |
HEX/VIC/JOE/TET |
Cy3 |
Texas red/TEX-615 |
Cy5 |
| Bio-Rad QX200 |
FAM |
HEX/VIC/JOE |
|
|
|
| Roche LightCycler 96 |
FAM |
HEX/VIC/JOE |
|
Texas red/TEX-615 |
Cy5 |
| Roche LightCycler 480 |
FAM |
HEX/VIC/JOE/TET |
|
Texas red/TEX-615 |
Cy5 |
|
