FITC

生物活性

FITC-Dextran (MW 4000) is a marker consisting of coupling fluorescein-isothiocyanate to dextran (4 kDa). FITC-Dextran are polysaccharides comprised of varying lengths of branched glucose molecules and can be used to determine solute, ion and protein permeability of the blood-brain barrier (BBB) based on the size of the dextran used^[1][2][3].

分子量

4000.00(Average)

CAS 号

60842-46-8

中文名称

荧光素异硫氰酸酯-葡聚糖(MW 4000)

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据

In Vitro: 

DMSO : 16.67 mg/mL (Need ultrasonic)

H₂O : 16.67 mg/mL (Need ultrasonic)

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% saline

  Solubility: 1.67 mg/mL (Infinity mM); Clear solution; Need ultrasonic

• 2.

  请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in saline)

  Solubility: 1.67 mg/mL (Infinity mM); Suspended solution; Need ultrasonic

• 3.

  请依序添加每种溶剂： 10% DMSO    90% corn oil

  Solubility: 1.67 mg/mL (Infinity mM); Clear solution; Need ultrasonic

*以上所有助溶剂都可在 MCE 网站选购。

参考文献

• [1]. Natarajan R, et al. Fluorescein Isothiocyanate (FITC)-Dextran Extravasation as a Measure of Blood-Brain Barrier Permeability. Curr Protoc Neurosci. 2017 Apr 10;79:9.58.1-9.58.15.

  [2]. Hoffmann A, et al. High and Low Molecular Weight Fluorescein Isothiocyanate (FITC)-Dextrans to Assess Blood-Brain Barrier Disruption: Technical Considerations. Transl Stroke Res. 2011 Mar;2(1):106-11.

  [3]. Eriksson I, et al. Analysis of Lysosomal pH by Flow Cytometry Using FITC-Dextran Loaded Cells. Methods Mol Biol. 2017;1594:179-189.