自从2011年ceRNA问世以来,关于ceRNA的文章出现了爆炸性的增长,无论是湿的,还是干的,特别是基于TCGA数据库构建的。我们找了一下相关的文章,TCGA中做能做的癌症类型貌似都被做了。不妨我们可以看一下: 1、Comprehensive analysis of lncRNA-associated ceRNA network in colorectal cancer(https:///10.1016/j.bbrc.2018.11.151) 2、Reconstruction and analysis of the aberrant lncRNA-miRNA-mRNA network based on competitive endogenous RNA in CESC (DOI: 10.1002/jcb.26850) 3、Construction and analysis of mRNA, miRNA,lncRNA, and TF regulatory networks reveal the key genes associated with prostate cancer(https:///10.1371/journal.pone.0198055) 4、Comprehensive analysis of a long noncoding RNA-associated competing endogenous RNA network in colorectal cancer(http://dx./10.2147/OTT.S158309) 5、Comprehensive analysis of competitive endogenous RNAs network associated with head and neck squamous cell carcinoma (DOI:10.1038/s41598-018-28957-y) 6、Comprehensive analysis of long noncoding RNA-associated competing endogenous RNA network in cholangiocarcinoma(https:///10.1016/j.bbrc.2018.10.186) 7、Identification of ceRNA network based on a RNA‑seq shows prognostic lncRNA biomarkers in human lung adenocarcinoma(DOI: 10.3892/ol.2018.9336) 8、Prediction of candidate RNA signatures for recurrent ovarian cancer prognosis by the construction of an integrated competing endogenous RNA network(DOI: 10.3892/or.2018.6707) 9、Identification of Six Potentially Long Noncoding RNAs as Biomarkers Involved Competitive Endogenous RNA in Clear Cell Renal Cell Carcinoma(https:///10.1155/2018/9303486) 。。。。 剩下的我们就不列出来了,实在太多了。 其实利用TCGA ceRNA这个套路也是非常简单的 1、下载TCGA的mRNA,miRNA,lncRNA 2、分别对mRNA,miRNA,lncRNA做差异分析 3、构建lncRNA-miRNA网络(网站预测,然后筛选出差异的lncRNA,miRNA) 4、构建miRNA-mRNA网络(网站预测,然后筛选出差异的miRNA,mRNA) 5、构建ceRNA |
|